Preservation of semen from Kintamani Bali dogs by freezing method

Puja, I Ketut; Sawitri, Ni Made; Maharani, Nisa; Heryani, Luh Gde Sri Surya; Dharmayudha, Anak Agung Gde Oka; Gunawan, I Wayan Nico Fajar

doi:10.5455/javar.2019.f326

Cited by 10 publications

(9 citation statements)

References 21 publications

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“…TherioSolution R declares that it possesses glucose extender in SA extender and chilling products and fructose in the chilling formula. Most of the extensors reported for use in cryopreservation of domestic canine semen contain either glucose, lactose, or fructose (16,18,(23)(24)(25)(26)(27)(28)(29)(30)(31)(32). Furthermore, fructose has been used intensively in extenders for wild dogs such as foxes (24)(25)(26)(27)33).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Chilled Dog Semen Extended With Sperm Activator

Salinas

2022

Front. Vet. Sci.

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Within modern biotechnology, different tools and methodologies have been developed to maximize canine semen conservation protocol to optimize reproductive results. In the last decades, the survival of chilled semen has been prolonged from 2 to 3 days with the first basic diluents, to 10–14 days with the modern extenders. However, their main limitation is that sperm quality decreases during cold storage. Sperm activators (SA) have been produced to provide the molecules necessary to maximize the sperm survival and quality with the aim to enhance fertility and prolificacy. In this study, the effect of commercial extender SA (Theriosolution® Canine AI extender -Chile-) was recorded by daily evaluation of chilled semen for 14 days. In this experiment, sperm-rich ejaculate fraction was collected from six adult healthy Neapolitan Mastiff dogs. The semen evaluation started immediately after collection (d0), and after that a next generation extender was added (d0) for every 24 h from d1 (with and without SA) to d14, to determine spermatozoa progressive motility, velocity of forward progression (VFP), morphology, and integrity of the spermatic membrane. The initial sperm concentration of extended semen was 417.3 ± 170.4 x 106/mL (mean ± SEM) with 85.89 ± 4.76% of MNS (morphologically normal sperm), 84.47 ± 5.22 % live sperm, and pH of 6.2 ± 2.8. The initial VFP was 3.83 ± 0.48, but after 1 min with SA, it rises to 4.45 ± 0.45 (P < 0.001). The sperm progressive motility parameter increases significantly (P < 0.05) in experimental trial, respect to control, starting to d2 at finish (except for d7). The VFP analysis significantly increases in experimental trial (P < 0.05) during most days of the study with the exclusion of d3 and d14. To evaluate the seminal characteristics over time, the experiment was divided into T1 (d0–d5), T2 (d6–d10), and T3 (d11–d14) (P < 0.001) in evaluation of morphology and membrane functionality. The MNS reached 70% at d10 and finally 65% at d14, being considered normal and possibly fertile. With Host-s, 65% of MNS were also achieved at d14. The presence of glucose and fructose in the diluents used for refrigeration can exert very important effects given the fact that metabolic routes have been found in both sugars, providing both different and complementing effects. It can be concluded that the use of SA prior to artificial insemination improves the quality of chilled semen significantly, although it does not reverse the effects of deterioration due to cellular metabolism over time.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Chilled Dog Semen Extended With Sperm Activator

Salinas

2022

Front. Vet. Sci.

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“…TherioSolution ® declares that it possesses glucose extender in SA extender and chilling products and also fructose in the chilling formula. Most of the extensors reported for use in cryopreservation of domestic canine sperm contain either glucose, lactose or fructose [15,19,[23][24][25][26][27][28][29][30][31][32]. Furthermore, fructose has been used intensively in extenders for wild dogs such as foxes [24][25][26][27]33].…”

Section: -Discussionmentioning

confidence: 99%

The effect of a canine semen activator supplementation or addiction on the long-term refrigeration quality of dog spermatozoa

2021

Preprint

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Within modern biotechnology, different tools have been developed to maximize canine semen conservation protocol to optimizing reproductive results and making their handling more flexible. In the last decades, the survival of refrigerated semen has been prolonged from 2-3d with the first basic diluents, to 10-14d using the most modern extenders. Semen activators ( SA ) have been produced to provide the molecules necessary to maximize the sperm survival and quality with the aim to enhance fertility and prolificacy. In this study, the effect of SA was recorded by daily evaluation of chilled semen 14d. For this experiment, Six adult healthy Neapolitan Mastiff dogs, were used as donors semen was manually collected. Spermatozoa-rich fractions of each subject was chilled using a new generation extender for long periods of time (d0) s tarting from the d1 to d1 4, different aliquot, with (experimental trial) and without SA (control trial), were evaluated daily for motility vigor, morphology and membrane integrity. The initial sperm concentration of extended semen was 417. 3±170 . 4x10 6 /mL (mean ± SEM) with 85.89±4.76% of MNS (morphologically normal sperm), 84.47±5.22 % vital sperm and a pH of 6.2±2.8. The initial vigor was 3.83±0.48, but after one min with SA , it rose to 4.45 ± 0.45 (P<0.001). The semen motility parameter increase significantly (P < 0.05) in experimental trial , respect to control, starting to d 2 at finish ( except for d 7). The vigor analysis significantly increase in experimental trial (P < 0.05) during the most day of the study with the exclusion of d 3 and d 14. For evaluate the semen characteristics over time, the experiment was divided into T1 (d0-d5), T2 (d6- d 10) and T3 (d11-d14) (P<0.001) in evaluation of morphology and membrane stability. The MNS reached 70% at d10 and finally 65% at −d14, being considered normal and possibly fertile. With Host-s, 65% of MNS were also achieved at d14. The presence of glucose and fructose in the diluents used for refrigeration can exert very important effects given the fact that metabolic routes have been found in both sugars, providing both different and complementing effects. It can be concluded that the use of SA prior to artificial insemination improves the quality of chilled semen significantly, although it does not reverse the effects of deterioration due to cellular metabolism over time.

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“…This was evident by a significant decrease in sperm motility and seminiferous tubular degeneration. Spermatozoa motility is an important attribute for assessing the fertility of spermatozoa (Puja et al, 2019). Motility is the main characteristic of live spermatozoa and the simplest parameter in the assessment of semen quality, the spermatozoa tail is the part that contains all the means needed to move (da Cunha et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Sperm Quality and Histology of the Testis and Epididymis in Chemical Castrated Male Cats with Intra-testicular Injection of Sodium Chloride Solution

Dayanti¹,

Berata²,

Puja³

2021

WVJ

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The high population of feral cats in Bali, Indonesia, could be a problem associated with cat welfare and the risk of zoonotic diseases. Gonadectomy or male castration is an option for population control; however, there would be more economical ways to reach this goal. Sterilization using chemicals is an alternative to the surgical method of castration as it is reasonably priced, activated in a short time, and does not affect animal activity after the chemical administration. The present study aimed to evaluate intratesticular injection of Sodium chloride as an agent of chemosterilants in male cats. In the current study, 16 healthy male cats aged nearly one year old were randomly selected. The male cats were allocated into four groups containing four cats in each. Group 1 (control) received bilateral intratesticular injection with 0.25 mL normal saline solution containing 2% Lidocaine. The cats in groups 2, 3, and 4 received bilateral intratesticular injection of 0.25 mL Sodium chloride solution containing 2% Lidocaine with a concentration of 15%, 20%, and 25%, respectively. At the end of the study (after 30 days of injection), the cats were castrated and their testes were removed for sperm quality and histopathological evaluation. The results of the present study indicated that intratesticular injection of Sodium chloride significantly decreased the motility and viability rates, and increased the abnormalities of the sperms. Histopathological studies revealed marked depletion of spermatozoa in the testes and seminiferous tubular degeneration, seminiferous tubular atrophy, and epithelial cell vacuolation. In conclusion, the injection of 20-25% solution of Sodium chloride in testes had the potential as a chemosterilant for chemical sterilization in cats.

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Preservation of semen from Kintamani Bali dogs by freezing method

Cited by 10 publications

References 21 publications

Evaluation of Chilled Dog Semen Extended With Sperm Activator

Evaluation of Chilled Dog Semen Extended With Sperm Activator

The effect of a canine semen activator supplementation or addiction on the long-term refrigeration quality of dog spermatozoa

Sperm Quality and Histology of the Testis and Epididymis in Chemical Castrated Male Cats with Intra-testicular Injection of Sodium Chloride Solution

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