Fasting samples of 914 subjects from healthy population were analyzed for total cholesterol, triglyceride and three major fractions of lipoproteins i.e. high-density lipoprotein cholesterol, low lipoprotein cholesterol and very low-density lipoprotein cholesterol. The values obtained were (in mg/dl) 165.7±30.2,88.36±31.2, 44.86±10.68, 101.66±29.8 and 18.11±7.35 respectively. When these subjects were grouped according to the age and sex, no appropriate differences were observed between most of the groups. Triglycerides were found to be low and HDL cholesterol was high in female when compared with male of similar age. Beyond age 40 years cholesterol level and low density lipoprotein cholesterol was found to be gradually increased in case of women. Minor difference was observed with dietary pattern. Present study suggests that clinical evaluation of patient should be made on the basis of these reference values for Western Maharashtra population.

Cerebrospinal fluid protein estimation is a routine diagnostic tool for various diseases. The proposed modified bromocresol green (BCG) method is applicable to automated as well as manual measurements. Protein concentrations and absorbance at 600 nm are linearly related throughout a wide range of concentrations from 10 mg % to 2000 mg %. Ninety C.S.F. samples were analyzed and results obtained by both the modified BCG method and routine pyrogallol red method were compared. An excellent co-relation was observed (n=90, g=0.996, P<0.001).

In the present study the property of lecUn agglutination in blood on normal and different canGer patients has been observed. The purified SynadenlumgranUimot I~'tin was non blood group specific and its utility as s diagnostic tool in mlignancy was studied. Hemagglutinstion (units/ml) of red blood ceils of different types of cancer was compared with the normal control's red blood cells. Out of 113 totil cancer patients, only a group of 29 breast cancer patients showed significant increase in ;;t,-e value (P<0.06) compared to normal control.

Twenty-four hour urinary albumin excretion (UAE) is considered as gold standard method for albuminuria measurement, but collection of 24-h urine is inconvenient. The aim of present study was to evaluate whether albumin: creatinine ratio (ACR) and urinary albumin concentration (UAC) in different spot urine samples correlate or not with 24-h UAE for screening of microalbuminuria in type 2 diabetic patients. We collected first morning void (FMV), random urine sample (RUS) and 24-h urine, separately on consecutive days from 104 type 2 diabetic patients. ACR and UAC in each spot urine sample compared with 24-h UAE with regard to Pearson correlation coefficient. Pearson's correlation of albumin: creatinine ratio (ACR) with 24-h UAE was (r = 0.802 and 0.623) in first morning void (FMV) and random urine sample (RUS), respectively. Pearson's correlation coefficient of urinary albumin concentration (UAC) compared with 24-h UAE was (r = 0.943 and 0.920), in FMV and RUS, respectively, P \ 0.01. Results revealed that values in first morning void (FMV) were better correlated with 24-h urinary albumin excretion (UAE), than the values in random urine sample (RUS). We conclude that the first morning void (FMV) may be able to replace 24-h urine collection, preferably urinary albumin concentration (UAC) in the initial screening of microalbuminuria in diabetic patients.

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The dry fruit extract of Tribulus terrestris has been used in traditional medicine for various therapeutic applications. This study was intended to investigate its anti- oxidant potential using in-vitro assays. Initially, the total polyphenolic, flavonoid, and tannin content of the ethanolic extract of its dry fruit were tested since these compounds confer majority of the reducing power to plant extracts. The anti- oxidant potential of the extract was investigated using radical scavenging assays such as DPPH and ABTS, superoxide radical scavenging assay, and ferric reducing anti-oxidant power assay. The polyphenol content was found to be 6.65 + 0.64 (Mean + S.E.M.; n = 3) mg GAE/ g of extract, flavonoid content was 0.40 + 0.02 (Mean + S.E.M.; n = 3) mg RE/ g of extract, and tannin content was 4.09 + 0.67 (Mean + S.E.M.; n = 3) mg TAE/ g of extract. The results for anti- oxidant assay obtained were found to be comparable to ascorbic acid and butylated hydroxytoluene standards. The results indicate presence of polyphenol compounds which confer majority of anti- oxidant properties to plant extracts. Further in-vitro and in-vivo investigations would reveal the efficacy of the extract as a dietary source of anti- oxidants