A sensitive and economical modified method for estimation of cerebrospinal fluid proteins

Durgawale, Pushpa; Kanase, Sudhir; Shukla, P. R.; Sontakke, Shubhangi

doi:10.1007/bf02867422

Cited by 11 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CSF cell count and analysis of biochemical parameters were performed for 19 cases (In one case pus sample was analysed). CSF glucose and protein levels were evaluated by automated methods using Hexokinase/Glucose‐6‐phosphate Dehydrogenase assay and Pyrogallol red assay respectively …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Central nervous system infection due to Cryptococcus gattii sensu lato in India: Analysis of clinical features, molecular profile and antifungal susceptibility

Mukhopadhyay

Bahubali

Netravathi

et al. 2017

Mycoses

View full text Add to dashboard Cite

Cryptococcus gattii species complex has evolved as a pathogen in the last two decades causing infection among both immunocompetent and immunocompromised hosts. We aimed to analyse the clinical features of CNS infection caused by C. gattii sensu lato, molecular and antifungal susceptibility profile of this pathogen. Cases diagnosed to have CNS cryptococcosis were included in the study. Cryptococcus recovered from patient's specimen was identified by standard protocol. Species confirmation, mating type and molecular type determination were performed by PCR based methods. Antifungal susceptibility was tested in VITEK2C to amphotericin B, 5-flucytosine, fluconazole and voriconazole. Among 199 cases, 20 (10%) were due to C. gattii, comprising of 75% cryptococcal meningitis and 25% cryptococcoma cases. Young adult males were commonly affected. Headache and vomiting were prominent symptoms and 50% were immunocompromised. Among the isolates, 75%, 20% and 5% were C. tetragattii, C. gattii sensu stricto and C. bacillisporus respectively and all had mating type α. Four (20%) isolates of C. tetragattii and the only isolate of C. bacillisporus were resistant to fluconazole. The most common species isolated from south India is C. tetragattii. The study contributes to the epidemiology of C. gattii and reiterates the need for genotyping and antifungal susceptibility testing.

show abstract

Section: Methodsmentioning

confidence: 99%

“…CSF glucose and protein levels were evaluated by automated methods using Hexokinase/Glucose-6-phosphate Dehydrogenase assay and Pyrogallol red assay respectively. 14…”

Section: Csf Analysismentioning

confidence: 99%

Central nervous system infection due to Cryptococcus gattii sensu lato in India: Analysis of clinical features, molecular profile and antifungal susceptibility

Mukhopadhyay

Bahubali

Netravathi

et al. 2017

Mycoses

View full text Add to dashboard Cite

show abstract

“…Soluble urokinase receptor values in CSF [33], PCT [34], CRP [35], matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 [36], and CD64 [37], correspond to the severity and outcome of purulent meningitis. It is a well-known fact that concentrations of albumins and the albumin coefficient are gold standard measures of BBB function [26,27,[38][39][40]. However, the data for purulent meningitis and predictive concentrations of albumins and the albumin coefficient in disease outcome are limited [32,40].…”

Section: Discussionmentioning

confidence: 99%

Blood-brain barrier damage as a predictor of outcome in patients with purulent meningitis

Dreshaj¹,

Shala²,

Ponosheci³

et al. 2018

Arch Med Sci Civil Dis

View full text Add to dashboard Cite

Introduction: Purulent meningitis is a severe infection of the central nervous system caused by different bacteria and very often causes severe complications and high lethality. Since the disease still has unclear pathogenesis and high mortality, researchers and clinicians are focused on the influence of damage of the blood-brain barrier (BBB) and its subsequent impact on the outcome of purulent meningitis. The aim of this study was to investigate the albumin concentration and the albumin coefficient as the best parameters of BBB damage, and their influence on the outcome of patients with purulent meningitis. Material and methods: The study was carried out by examining the hospitalized patients in the Clinic of Infective Diseases in Prishtina, Kosovo. Blood-brain barrier damage is evaluated by analyzing the dynamics of values of proteins, glucose and albumins, as well as by determining the BBB coefficient of damage. Results: The data consistent with BBB damage in patients with purulent meningitis corresponded with disease outcome. Consequently, 56.97% of the patients who developed complications or died had very severe BBB damage. Patients with the mean value of albumin coefficient X = 46.5 ±27.23 g/l were at higher risk of developing complications (relative risk (RR) = 2.63; p < 0.0001) or having a lethal outcome (RR = 5.20; p < 0.0001). Patients with longer duration of pathological mean values of albumin coefficient were at higher risk of developing complications (RR = 3.11; p < 0.0001). Conclusions: Our data suggest that BBB damage in patients with purulent meningitis correspond with disease outcome. The best predictors for outcome were albumin coefficient and albumin concentration in cerebrospinal fluid.

show abstract

“…When bromochresol green (BCG) was added directly to the media, 0.0015% was used. We used both BCG preincorporated into the media as well as post incubation addition of a BCG dye reagent for 1–2 hrs containing 0.028% BCG dissolved in 0.56% (w/v) succinic acid, 0.1% (w/v) NaOH (Vijayaraghavan and Vincent; 2013) with 0.6% Brij-35 (Durgawale et al, 2005) acidified to pH 4.2 with HCl. This dye reagent was utilized following exposure to a protease inhibitor or bacterial growth and subsequent exposure to a protease.…”

Section: Methodsmentioning

confidence: 99%

A culture-based method for determining the production of secreted protease inhibitors

Quintero

Bermudes

2014

Journal of Microbiological Methods

View full text Add to dashboard Cite

We have developed a culture-based method for determining the production of secreted protease inhibitors. The assay utilizes standard proteolysis detection plates to support microbial growth followed by infiltrating the plate with a protease and subsequently detecting the remaining protein by trichloroacetic acid (TCA) precipitation, or by bromocreosol green (BCG) or Ponseau S (PS) staining. The presence of a protease inhibitor can be observed in the form of a protected zone of protein around the protease inhibitor-producing strain. Using the protease inhibitors α-2-macroglobulin, aprotinin, leupeptin, and bestatin and the primary and secondary forms of Photorhabdus luminescens in combination with the protease trypsin, we were able to demonstrate that the assay is specific for the cognate inhibitor of the protease and for bacteria secreting protease inhibitors. In addition, when casein-containing plates were used, the size of the diffusion zone was inversely correlated with the molecular weight of the inhibitor allowing a relative estimation of the protease inhibitor molecular weight. This assay is useful for detecting the presence of microbial secreted protease inhibitors and may reveal their production by microorganisms that were not previously recognized to produce them.

show abstract

A sensitive and economical modified method for estimation of cerebrospinal fluid proteins

Cited by 11 publications

References 7 publications

Central nervous system infection due to Cryptococcus gattii sensu lato in India: Analysis of clinical features, molecular profile and antifungal susceptibility

Central nervous system infection due to Cryptococcus gattii sensu lato in India: Analysis of clinical features, molecular profile and antifungal susceptibility

Blood-brain barrier damage as a predictor of outcome in patients with purulent meningitis

A culture-based method for determining the production of secreted protease inhibitors

Contact Info

Product

Resources

About