The present study examined the antioxidant activity of conjugated octadecatrienoic fatty acid (9 cis,11 trans,13 trans-18:3), alpha-eleostearic acid, of karela seed (Momordica charantia), fed to rats for 4 wk. The growth pattern of rats and the effect on plasma cholesterol and high density lipoprotein (HDL) cholesterol and peroxidation of plasma lipid, lipoprotein, eryhrocyte membrane, and liver lipid were measured. Rats were raised on diets containing sunflower oil mixed with three different levels of conjugated trienoic fatty acid (9c,11t,3t-18:3) 0.5, 2, and 10% by weight; the control group was raised with sunflower oil as dietary oil as the source of linoleic acid (9c,12c-18:2). The growth pattern of the three experimental groups of rats showed no significant difference compared to the control group of rats, but the group with 10% 9c,11t,13t-18:3 had slightly higher body weight than the control group of rats. Concentrations of total cholesterol, HDL-cholesterol, and non-HDL-cholesterol in plasma were similar in all four groups. Plasma lipid peroxidation was significantly lower in the case of 0.5% 9c,11t,13t-18:3 group than the control group and the 2 and 10% 9c,11t,13t-18:3 dietary groups as well. Lipoprotein oxidation susceptibility test with 0.5, 2, and 10% 9c,11t,13t-18:3 dietary groups was significantly less susceptible to lipoprotein peroxidation when compared with sunflower oil dietary group, and the dietary group with 0.5% 9c,11t,13t-18:3 showed least susceptibility. There was significant lowering in erythrocyte ghost membrane lipid peroxidation in the 0.5, 2, and 10% 9c,11t,13t-18:3 dietary groups compared to the sunflower oil groups. Nonenzymatic liver tissue lipid peroxidation was significantly lower in the group of rats raised on 0.5% 9c,11t,13t-18:3, but the groups on 2 and 10% 9c,11t,13t-18:3 acid did not show any significant difference compared with the control group of rats.

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Polyphenolic extracts (PE) of edible flower of Sesbania grandiflora were tested to evaluate its antimicrobial effect against some common pathogenic bacteria and growth promoting property against probiotic organism Lactobacillus acidophilus. The antimicrobial activity of S. grandiflora flower PE against selected pathogens was evaluated using both in vitro and in situ methods. In vitro studies suggested that PE has inhibitory effect against Staphylococcus aureus, Shigella flexneri 2a, Salmonella Typhi, Escherichia coli and Vibrio cholerae. The gram-positive organism S. aureus was the most sensitive organism to PE and minimum inhibitory concentration (MIC) was found to be 0.013 mg/mL where as the MIC of PE against V. cholerae was the highest (0.25 mg/mL). On the other hand PE showed growth promoting effect on the common probiotic bacterium L. acidophilus. The major finding was that S. grandiflora PE induced a significant biomass increase of L. acidophilus grown in liquid culture media. PE showed reduction of S. aureus growth in food (fish) during storage at 10°C. High performance liquid chromatography analysis showed that rutin, a major flavonoid of the PE diminished in the culture medium MRS broth with the growth of L. acidophilus.

Three different cultivars of marigold flowers ( Tagetes patula L.) (marigold orange, marigold yellow, and marigold red) were analyzed for the lutein ester contents, and the in vitro antioxidative activities of the flower extracts were compared. The total antioxidant capacity, reducing power, hydroxyl, DPPH, and ABTS(*+) radical scavenging activities, iron chelation capacity, and inhibition of lipid peroxidation in a linoleic acid emulsion system were measured. Iron-mediated Fenton reaction was carried out to evaluate the protective effect of leutin against DNA damage. The marigold orange (MGO) variety contains the maximum amount of lutein. It also had the highest DPPH radical scavenging activity and ABTS radical scavenging activity, with an EC(50) value of 0.344 mg/mL. It was also the most effective against lipid peroxidation and hydroxyl radical scavenging activities. The MGO extract has the maximum reducing power. Hepatic cell damage in iron-mediated Fenton reaction caused by free radicals was reduced by the marigold extracts. Marigold flowers of Indian variety can be effectively utilized to produce lutein ester, which can be used as a food supplement or as an accessible source of natural antioxidants.

The nutritive value of conjugated octadecatrienoic fatty acid (C18:3 Δ9,11,13α-elaeostearic acid) as occurs in karela seed (Momordica charantia) oil was studied. When rats were fed at the 20% level for 6 weeks, the growth pattern of rats and the food efficiency ratio showed a slightly significant difference compared to the group raised on linseed oil with non-conjugated octadecatrienoic acid (C18:3 Δ9,12,15α-linolenic acid) as control. The concentrations of total cholesterol, triglyceride, VLDL-cholesterol, LDL-cholesterol and LDL/HDL-cholesterol in serum were significantly higher in karela oil than in linseed oil. The liver lipid profile showed no difference. The total lipid as well as phospholipid concentrations of heart lipid were significantly higher in karela oil. The brain weight of the linseed oil group was significantly higher than the karela group. The brain phospholipid concentration of the karela oil group was significantly higher than that of the linseed oil group.

The present study investigated the dietary effect of conjugated linolenic acid (CLnA) on lipid profiles and lipid peroxidations in alloxan-induced diabetes mellitus in rats. Diabetic rats were fed with 20% sunflower oil (diabetic control), sunflower oil supplemented with 0.5% CLnA, sunflower oil supplemented with 0.15% alpha-tocopherol, and sunflower oil containing 0.25% CLnA + 0.15% alpha-tocopherol. The results demonstrated that 0.5% CLnA, 0.15% alpha-tocopherol, and 0.25% CLnA + 0.15% alpha-tocopherol each on supplementation significantly lowered total cholesterol and non-HDL-cholesterol in comparison with the diabetic control group. The TAG level was significantly lowered in both the 0.15% alpha-tocopherol and 0.25% CLnA + 0.15% alpha-tocopherol groups. LDL-lipid peroxidation and erythrocyte membrane lipid peroxidation were reduced significantly in each of the experimental groups vs. the control group. The CLnA + alpha-tocopherol diet induced a greater reduction in membrane lipid and liver lipid peroxidation than the alpha-tocopherol diet alone. In conclusion, dietary CLnA exerts antioxidant activity as evidenced by reduced lipid peroxidation in chemically induced diabetes mellitus.

There is increasing interest in the use of natural preservatives (rather than synthetic ones) for maintaining the quality and safety of foods due to their perceived environmental and health benefits. In particular, plant-based antimicrobials are being employed to protect against microbial spoilage, thereby improving food safety, quality, and shelf-life. However, many natural antimicrobials cannot be utilized in their free form due to their chemical instability, poor dispersibility in food matrices, or unacceptable flavor profiles. For these reasons, encapsulation technologies, such as nanoemulsions, are being developed to overcome these hurdles. Indeed, encapsulation of plant-based preservatives can improve their handling and ease of use, as well as enhance their potency. This review highlights the various kinds of plant-based preservatives that are available for use in food applications. It then describes the methods available for forming nanoemulsions and shows how they can be used to encapsulate and deliver plant-based preservatives. Finally, potential applications of nano-emulsified plant-based preservatives for improving food quality and safety are demonstrated in the meat, fish, dairy, and fresh produce areas.