Dietary comparison of conjugated linolenic acid (9 cis, 11 trans, 13 trans) and α‐tocopherol effects on blood lipids and lipid peroxidation in alloxan‐induced diabetes mellitus in rats

Abstract

The present study investigated the dietary effect of conjugated linolenic acid (CLnA) on lipid profiles and lipid peroxidations in alloxan-induced diabetes mellitus in rats. Diabetic rats were fed with 20% sunflower oil (diabetic control), sunflower oil supplemented with 0.5% CLnA, sunflower oil supplemented with 0.15% alpha-tocopherol, and sunflower oil containing 0.25% CLnA + 0.15% alpha-tocopherol. The results demonstrated that 0.5% CLnA, 0.15% alpha-tocopherol, and 0.25% CLnA + 0.15% alpha-tocopherol each on supplementation significantly lowered total cholesterol and non-HDL-cholesterol in comparison with the diabetic control group. The TAG level was significantly lowered in both the 0.15% alpha-tocopherol and 0.25% CLnA + 0.15% alpha-tocopherol groups. LDL-lipid peroxidation and erythrocyte membrane lipid peroxidation were reduced significantly in each of the experimental groups vs. the control group. The CLnA + alpha-tocopherol diet induced a greater reduction in membrane lipid and liver lipid peroxidation than the alpha-tocopherol diet alone. In conclusion, dietary CLnA exerts antioxidant activity as evidenced by reduced lipid peroxidation in chemically induced diabetes mellitus.

Antioxidative Effect of Rice Bran Oil and Medium Chain Fatty Acid Rich Rice Bran Oil in Arsenite Induced Oxidative Stress in Rats

“…Then, 10 ml of chloroform was added and homogenization continued for another 30 s. Finally, 10 ml of redistilled water were added and the mixture was homogenised for 30 s. The tubes were then centrifuged for 20 min at 4000 rpm, and the chloroform layer was separated 27 . Thiobarbituric acidreactive substances were measured according to the method described by Schmedes & Hølmer 28 . Malondialdehyde MDA concentration was calculated by taking the extinction coefficient of MDA to be 1.56 10 5 /M cm 29 .…”

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“…Alternatively, conjugated double bonds of PA and other CLnAs in PSO entrapped free radicals 71 . Membrane lipid peroxidation was decreased by diet containing 0.25 % CLnAs compare to diet without CLnAs 72 . Lowered oxidative stress and enhanced antioxidant enzyme serum levels were observed in Saha and Ghosh study.…”

Comparative prophylactic effects of α-eleostearic acid rich nano and conventional emulsions in induced diabetic rats

“…Although there was a significant rise in GSH levels in 0.5 % BGO-NE diet supplemented group than 1 % BGO NE, 0.5 % BGO-CE and 1 % BGO-CE formulated diet supplemented group in plasma, there was no significant difference between the BGO nanoemulsion supplemented groups in GSH levels in liver homogenate. This can be explained by the role of 9 c, 11 t, 13 t -18:3 conjugated octadecatrienoic fatty acids (ESA) as described by Dhar et al (2006) as free radical scavengers present in the investigated in vivo system. Alloxan, cytotoxic glucose analogue exerts its diabetogenic pathological manifestations by specifically inhibiting glucokinase (glucose sensor of β cell) causing selective β cell necrosis which leads to insulin-dependent diabetes mellitus.…”

“…Previous studies indicated oral administration of α-eleostearic acid (ESA) (present in BGO) worked optimally at 0.5 % of the oil fed to the in vivo systems in conferring health benefits (Dhar et al 2006). Thus, a rationale behind this attempt also entailed to evaluate the efficiency of ESA in the formulated NE compared to CE system on in vivo oxidative stressed system at lower dosage (∼0.25 %) than 0.5 % (as has been established previously).…”