Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus

Abstract

Polyphenolic extracts (PE) of edible flower of Sesbania grandiflora were tested to evaluate its antimicrobial effect against some common pathogenic bacteria and growth promoting property against probiotic organism Lactobacillus acidophilus. The antimicrobial activity of S. grandiflora flower PE against selected pathogens was evaluated using both in vitro and in situ methods. In vitro studies suggested that PE has inhibitory effect against Staphylococcus aureus, Shigella flexneri 2a, Salmonella Typhi, Escherichia coli and Vibrio cholerae. The gram-positive organism S. aureus was the most sensitive organism to PE and minimum inhibitory concentration (MIC) was found to be 0.013 mg/mL where as the MIC of PE against V. cholerae was the highest (0.25 mg/mL). On the other hand PE showed growth promoting effect on the common probiotic bacterium L. acidophilus. The major finding was that S. grandiflora PE induced a significant biomass increase of L. acidophilus grown in liquid culture media. PE showed reduction of S. aureus growth in food (fish) during storage at 10°C. High performance liquid chromatography analysis showed that rutin, a major flavonoid of the PE diminished in the culture medium MRS broth with the growth of L. acidophilus.

Eruca sativa Might Influence the Growth, Survival under Simulated Gastrointestinal Conditions and Some Biological Features of Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus Strains

“…Generally, the presence of plant extracts does not affect the growth of microorganisms [20,21], but in some cases, they can benefit from the presence of these bio-components, increasing, for example, their survival during gastro-intestinal transit. This effect is influenced by both the type of strain and by the vegetable matrix [22].…”

Antiproliferative and Apoptotic Effects ofSesbania grandifloraLeaves in Human Cancer Cells

“…It has also been shown that the roots of S. grandiflora possess antituberculosis activity [7]. The flower extracts of this plant have also been proved to possess antimicrobial activities [8]. In one of our previous studies we have found that the leaves of this plant possess protective roles against rat kidney during alcohol and polyunsaturated fatty acid induced oxidative stress [3].…”

Experimental studies on bioactive potential of rutin

“…[26] A number of plant extracts rich in flavonoids are known possess antimicrobial, anthelmintic, and cytotoxic potential. [27][28][29][30][31][32][33][34][35][36] The diverse therapeutic potential of plant flavonoids is due to their chemical structures. Such phytochemicals from plants serve as important therapeutic agents.…”