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Background. The most common species isolated from primary endodontic infections are black-pigmented bacteria. These species are implicated in apical abscess formation due to their proteolytic activity and are fastidious in nature. Therefore, the present study was carried out to evaluate the presence and identification of various pigmented Porphyromonas and Prevotella species in the infected root canal through culture-based techniques.Methods. Thirty-one patients with primary endodontic infections were selected. Using sterile paper points, samples were collected from the root canals after access opening and prior to obturation, which were cultured using blood and kanamycin blood agar. Subsequently, biochemical test was used to identify the species and the results were analyzed using percentage comparison analysis, McNemar and chi-squared tests, Wilcoxon match pair test and paired t-test.Results. Out of 31 samples 26 were positive for black-pigmented organisms; the predominantly isolated species were Prevotella followed by Porphyromonas. In Porphyromonas only P. gingivalis was isolated. One of the interesting features was isolation of P. gingivalis through culture, which is otherwise very difficult to isolate through culture.Conclusion. The presence of Prevotella and Porphyromonas species suggests that a significant role is played by these organisms in the pathogenesis of endodontic infections.

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Background: Among the many oral bacteria residing in the oral cavity, Porphyromonas gingivalis is known to be the major organism associated with periodontitis. Different methods have been used for identification. However, culture is the gold standard in identification. Culture helps in characterization of the physiologic characters, identification pathogenic traits, virulence factors, etc. Gene alterations can be studied better if the organism is cultivated in the laboratory. Aims and Objectives: The aims and objectives were to study the isolation of P. gingivalis from subgingival plaque samples of periodontally healthy and diseased individuals by culture and phenotypic identification. Materials and Methods: A total of 400 subjects each with 200 chronic periodontitis and healthy individuals were selected. Patients meeting all criteria's samples were inoculated on Blood agar fortified with Hemin and Vitamin K media. Phenotypic characterization was done by morphological and biochemical analysis to identify P. gingivalis. Results: Of 400 samples, 173 were male and 227 were female. In chronic periodontitis, 119 were female and 81 were male. The total sample positive for P gingivalis was 288. In chronic periodontitis, the positivity for P gingivalis was 179. The positivity for P gingivalis was more in females 168. Conclusion: Identification of anaerobic microbes can be easily done these anaerobic culture techniques. Hence, we recommend using these techniques in the identification of oral anaerobes as a routine laboratory procedure which is lacking in our country.

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