Prevalence of Porphyromonas gingivalis isolated from oral cavity in Indian population - A culture-based study

Abstract

Background: Among the many oral bacteria residing in the oral cavity, Porphyromonas gingivalis is known to be the major organism associated with periodontitis. Different methods have been used for identification. However, culture is the gold standard in identification. Culture helps in characterization of the physiologic characters, identification pathogenic traits, virulence factors, etc. Gene alterations can be studied better if the organism is cultivated in the laboratory. Aims and Objectives: The aims and objectives were to study the isolation of P. gingivalis from subgingival plaque samples of periodontally healthy and diseased individuals by culture and phenotypic identification. Materials and Methods: A total of 400 subjects each with 200 chronic periodontitis and healthy individuals were selected. Patients meeting all criteria's samples were inoculated on Blood agar fortified with Hemin and Vitamin K media. Phenotypic characterization was done by morphological and biochemical analysis to identify P. gingivalis. Results: Of 400 samples, 173 were male and 227 were female. In chronic periodontitis, 119 were female and 81 were male. The total sample positive for P gingivalis was 288. In chronic periodontitis, the positivity for P gingivalis was 179. The positivity for P gingivalis was more in females 168. Conclusion: Identification of anaerobic microbes can be easily done these anaerobic culture techniques. Hence, we recommend using these techniques in the identification of oral anaerobes as a routine laboratory procedure which is lacking in our country.

Porphyromonas gingivalis and its impact on periodontal health and systemic diseases. A concise review.

“…Rojas et al (2017) found a prevalence of 73.3% in adult patients with diagnosed periodontal disease. Ingalagi et al (2018) found a high prevalence in healthy adult patients, as well as patients with periodontal disease. The prevalence of P. gingivalis was high in the group of healthy patients with 179 subjects (89.5%) compared to 109 subjects (54.0%) in the group of patients with periodontal disease.…”