No abstract

A pale-yellowish bacterium, strain KWS-1T, was isolated from seawater during a study of the bacterial diversity of the marine environment of the Kanyakumari coastal region of the Bay of Bengal, India, and was studied by using a polyphasic taxonomic approach. Strain KWS-1T had morphological and chemotaxonomic properties (cell-wall diamino acid, menaquinone and fatty acid profile) consistent with its classification in the genus Brachybacterium. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain KWS-1T was related most closely to Brachybacterium paraconglomeratum JCM 17781T, followed by Brachybacterium saurashtrense DSM 23186T, Brachybacterium gingengisoli JCM 19356T, Brachybacterium faecium JCM 11609T and Brachybacterium conglomeratum JCM 11608T (98.45, 98.24, 98.12, 98.10 and 98.10 % similarity, respectively), whereas the sequence similarity values with respect to the other Brachybacterium species with validly published names were between 97.4 and 94.2 %. However, the DNA-DNA hybridization values between strain KWS-1T and the five most closely related species were less than the threshold value for species discrimination. The major lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine and the major quinone was menaquinone MK-7. The DNA G+C content of strain KWS-1T was 71.8 mol%. The above data in combination with the phenotypic distinctiveness of strain KWS-1T from other reference strains clearly indicate that the strain represents a novel species, for which the name Brachybacterium aquaticum sp. nov. is proposed. The type strain is KWS-1T (=MTCC 11836T=DSM 28796T=JCM 30059T).

A novel bacterium (strain NIO-1002 T ) belonging to the genus Microbacterium was isolated from a marine sediment sample in Chorao Island, Goa Province, India. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Cells of this strain were Gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. It grew in 0-12 % (w/v) NaCl and at 25-37 8C, with optimal growth at 30 8C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1002 T is associated with members of the genus Microbacterium, with highest sequence similarity with et al., 2011). In this study, we describe a novel isolate belonging to the genus Microbacterium recovered from a sediment sample collected from Chorao Island, Goa, India.Strain NIO-1002 T was isolated from a marine sediment sample taken from Chorao Island (GPS coordinates 158 329 340 N 738 559 150 E) isolated on marine agar (MA) using the standard serial dilution method. The MA plates were (Hi-Media, Mumbai) incubated at 30 8C for 3-4 days. Isolated colonies were purified by repeated streaking on MA. The purified strain was maintained on MA slants at 4 8C and as glycerol suspensions (20 %, v/ v) at 280 8C. Biomass for chemotaxonomy and molecular-systematic studies was obtained following growth in shake flasks (about 200 r.p.m.) of tryptic soy broth at 30 8C for 5 days. Gram staining was carried out using the standard Gram reaction and cell motility was confirmed

A Gram-stain-positive bacterium, designated strain NIO-1109 T , was isolated from a marine sediment sample from Chorao Island, Goa, India.

Two novel Gram-stain-negative, slow-growing, halotolerant strains with rod-shaped cells, designated as strains Mi-7 T and Mi-8, which formed pinpoint colonies on halophilic media were isolated during a study into the microbial diversity of a salt pan in the state of Tamilnadu, India. Both the strains had an obligate requirement for 1 % (w/v) NaCl for growth and were halotolerant, growing at NaCl concentrations of up to 20 % (w/v) in media. The strains, however, showed an inability to utilize the majority of substrates tested as sole carbon sources for growth and in fermentation reactions. Molecular phylogenetic analyses, based on 16S rRNA gene sequence revealed their closest phylogenetic neighbours to be members of the genus Marinobacter, with whom they showed the highest sequence similarity of 93.6 % and even less with the type strain of the type species, Marinobacter hydrocarbonoclasticus DSM 8798 T (91.1 %). Similarities with other genera within the family Alteromonadaceae were below 91.0 %. However, the two strains were very closely related to each other with 99.9 % sequence similarity, and DNA-DNA hybridization analyses confirmed their placement in the same species. The DNA G+C content of both strains was 65 mol%. Using the polyphasic taxonomic data obtained from this study, strains Mi-7 T and Mi-8 represent two strains of the same species of a novel genus for which the name Tamilnaduibacter salinus gen. nov., sp. nov., is proposed; the type strain of the novel species is Mi-7 T (5MTCC 12009 T 5DSM 28688 T).

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated SGD-V-25 T was isolated from Veraval sediment sample, India. Strain SGD-V-25T was capable of growing at 25-50 6C (optimum 37 6C), pH 6-12 (optimum pH 7.0) and with 0-5 % (w/v) NaCl. The taxonomic position of this strain was deduced using a polyphasic approach and the 16S rRNA gene sequence analysis showed that the isolate belongs to the phylum Firmicutes, forming the cluster with Bacillus badius MTCC 1548 T , with which it shares highest similarity of 99.1 % with 13 nt differences. Other type strains of the genus Bacillus showed less than 96 % similarity. The cell wall contained mesodiaminopimelic acid as the diagnostic diamino acid. The polar lipid profile of strain SGD-V-25 T showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phsophoglycolipid and two aminophospholipids. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 16 : 0 , C 16 : 1 v11c and C 16 : 0. The genomic DNA G+C content of strain SGD-V-25 T was 37.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA hybridization, strain SGD-V-25 T could be clearly distinguished from closely related members of the genus Bacillus, and the name Bacillus encimensis sp. nov., is proposed to accommodate this strain. The type strain is SGD-V-25 T (5NCIM 5513 T 5DSM 28241 T ).

Beauveria bassiana is an insect-associated fungal species and it is economically important as an environment-friendly mycoinsecticide and a good source of biologically active secondary metabolites. In this study, we employed DNA microsatellite markers to investigate the infraspecific diversity of 102 isolates of B. bassiana sensu stricto from India. All the microsatellite markers were easily amplifiable for all the isolates and provided a good genotype resolution. The microsatellite analysis indicated the presence of highly polymorphic, randomly distributed populations of B. bassiana with variable host range and apparently no host-specificity. We, however, observed region-wise clustering of the populations within B. bassiana sensu stricto from India.

No abstract