Bacillus encimensis sp. nov. isolated from marine sediment

Dastager, Syed G.; Mawlankar, Rahul; Mual, Poonam; Verma, Ashish; Krishnamurthi, Srinivasan; Joseph, Neetha; Shouche, Yogesh S.

doi:10.1099/ijs.0.000114

Cited by 19 publications

(4 citation statements)

References 33 publications

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“…Besides, Clostridium sensu stricto 1 and Clostridium sensu stricto 10 appeared in SI-0 with the RAs of 25.10 and 15.27%, respectively. Members of Bacillus are aerobes or facultative anaerobes that produce cellulase, hemicellulase, ligninase, amylase, protease, and pectinase ( Dastager et al, 2015 ; Yang et al, 2017 ; Xu et al, 2018 ). Therefore, this genus might result in the rapid consumption of oxygen during the pretreatment (as shown in Figure 2B ) and induce cellulose and lignin degradation.…”

Section: Resultsmentioning

confidence: 99%

Anaerobic and Microaerobic Pretreatment for Improving Methane Production From Paper Waste in Anaerobic Digestion

Song

Cai

et al. 2021

Front. Microbiol.

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Having been generated with a tremendous amount annually, paper waste (PW) represents a large proportion in municipal solid waste (MSW) and also a potential source of renewable energy production through the application of anaerobic digestion (AD). However, the recalcitrant lignocellulosic structure poses obstacles to efficient utilization in this way. Recently, anaerobic and microaerobic pretreatment have attracted attention as approaches to overcome the obstacles of biogas production. This study was set out to present a systematic comparison and assessment of anaerobic and microaerobic pretreatment of PW with different oxygen loadings by five microbial agents: composting inoculum (CI), straw-decomposing inoculum (SI), cow manure (CM), sheep manure (SM), and digestate effluent (DE). The hints of microbial community evolution during the pretreatment and AD were tracked by 16S rRNA high-throughput sequencing. The results demonstrated that PW pretreated by DE with an oxygen loading of 15 ml/gVS showed the highest cumulative methane yield (CMY) of 343.2 ml/gVS, with a BD of 79.3%. In addition to DE, SI and SM were also regarded as outstanding microbial agents for pretreatment because of the acceleration of methane production at the early stage of AD. The microbial community analysis showed that Clostridium sensu stricto 1 and Clostridium sensu stricto 10 possessed high relative abundance after anaerobic pretreatment by SI, while Bacteroides and Macellibacteroides were enriched after microaerobic pretreatment by SM, which were all contributable to the cellulose degradation. Besides, aerobic Bacillus in SI and Acinetobacter in SM and DE probably promoted lignin degradation only under microaerobic conditions. During AD, VadinBC27, Ruminococcaceae Incertae Sedis, Clostridium sensu stricto 1, Fastidiosipila, and Caldicoprobacter were the crucial bacteria that facilitated the biodegradation of PW. By comparing the groups with same microbial agent, it could be found that changing the oxygen loading might result in the alternation between hydrogenotrophic and acetoclastic methanogens, which possibly affected the methanogenesis stage. This study not only devised a promising tactic for making full use of PW but also provided a greater understanding of the evolution of microbial community in the pretreatment and AD processes, targeting the efficient utilization of lignocellulosic biomass in full-scale applications.

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Section: Resultsmentioning

confidence: 99%

Anaerobic and Microaerobic Pretreatment for Improving Methane Production From Paper Waste in Anaerobic Digestion

Song

Cai

et al. 2021

Front. Microbiol.

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“…Effect of temperature on growth of P1 was determined by incubating culture at various temperatures from 25-75 C. Effect of NaCl concentration on growth of P1 was determined at various NaCl concentrations of 0-10%. Effect of incubation period on growth of P1 was determined by observing its growth rate up to 120 h [3,4,35,36,37,38,39].…”

Section: Preliminary Identification Of P1 Isolatementioning

confidence: 99%

Enhanced catalytic activity of Bacillus aryabhattai P1 protease by modulation with nanoactivator

Pathak

Rathod

Mahabole

et al. 2020

Heliyon

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In the developing area of modern nanobiotechnology, the research is being focused on enhancement of catalytic performance in terms of efficiency and stability of enzymes to fulfill the industrial demand. In the context of this interdisciplinary era, we isolated and identified alkaline protease producer Bacillus aryabhattai P1 by polyphasic approach and then followed one variable at a time approach to optimize protease production from P1. The modified components of fermentation medium (g/L) were wheat bran 10, soybean flour 10, yeast extract 5, NaCl 10, KH 2 PO 4 1, K 2 HPO 4 1 and MgSO 4 ⋅7H 2 O 0.2 (pH 9). The optimum alkaline protease production from P1 was recorded 75 AE 3 U/mg at 35 C and pH 9 after 96 h of fermentation period. Molecular weight of partially purified P1 alkaline protease was 26 KDa as revealed by SDS-PAGE. Calcium based nanoceramic material was prepared by wet chemical precipitation method and doped in native P1 protease for catalytic activity enhancement. Catalytic activity of modified P1 protease was attained by nanoactivator mediated modulation was more by 5.58 fold at pH 10 and 30 C temperature. The nanoceramic material named as nanoactivator, with grain size of 40-60 nm was suitable to redesign the active site of P1 protease. Such types of modified proteases can be used in different nanobiotechnological applications.

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“…Bacillus species are ubiquitous Gram-positive bacteria known for their ability to survive in a wide variety of environments, including marine environments such as seawater 1 , tidal flat 2–4 , and sediments 5–10 ; soil environments such as rhizospheres 11–15 ; human gut samples 16–18 ; as well as food samples such as dairy products 19,20 and fermented soybeans 19 . For several Bacillus strains from such diverse environments, in silico methods 21,22 and in vitro experiments 23–30 have shown strong biosynthetic and superb protein secreting capabilities.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics study reveals Red Sea Bacillus with characteristics associated with potential microbial cell factories (MCFs)

Othoum

Prigent

Derouiche

et al. 2019

Sci Rep

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Recent advancements in the use of microbial cells for scalable production of industrial enzymes encourage exploring new environments for efficient microbial cell factories (MCFs). Here, through a comparison study, ten newly sequenced Bacillus species, isolated from the Rabigh Harbor Lagoon on the Red Sea shoreline, were evaluated for their potential use as MCFs. Phylogenetic analysis of 40 representative genomes with phylogenetic relevance, including the ten Red Sea species, showed that the Red Sea species come from several colonization events and are not the result of a single colonization followed by speciation. Moreover, clustering reactions in reconstruct metabolic networks of these Bacillus species revealed that three metabolic clades do not fit the phylogenetic tree, a sign of convergent evolution of the metabolism of these species in response to special environmental adaptation. We further showed Red Sea strains Bacillus paralicheniformis (Bac48) and B. halosaccharovorans (Bac94) had twice as much secreted proteins than the model strain B. subtilis 168. Also, Bac94 was enriched with genes associated with the Tat and Sec protein secretion system and Bac48 has a hybrid PKS/NRPS cluster that is part of a horizontally transferred genomic region. These properties collectively hint towards the potential use of Red Sea Bacillus as efficient protein secreting microbial hosts, and that this characteristic of these strains may be a consequence of the unique ecological features of the isolation environment.

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Bacillus encimensis sp. nov. isolated from marine sediment

Cited by 19 publications

References 33 publications

Anaerobic and Microaerobic Pretreatment for Improving Methane Production From Paper Waste in Anaerobic Digestion

Anaerobic and Microaerobic Pretreatment for Improving Methane Production From Paper Waste in Anaerobic Digestion

Enhanced catalytic activity of Bacillus aryabhattai P1 protease by modulation with nanoactivator

Comparative genomics study reveals Red Sea Bacillus with characteristics associated with potential microbial cell factories (MCFs)

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