Brachybacterium aquaticum sp. nov., a novel actinobacterium isolated from seawater

Abstract

A pale-yellowish bacterium, strain KWS-1T, was isolated from seawater during a study of the bacterial diversity of the marine environment of the Kanyakumari coastal region of the Bay of Bengal, India, and was studied by using a polyphasic taxonomic approach. Strain KWS-1T had morphological and chemotaxonomic properties (cell-wall diamino acid, menaquinone and fatty acid profile) consistent with its classification in the genus Brachybacterium. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain KWS-1T was related most closely to Brachybacterium paraconglomeratum JCM 17781T, followed by Brachybacterium saurashtrense DSM 23186T, Brachybacterium gingengisoli JCM 19356T, Brachybacterium faecium JCM 11609T and Brachybacterium conglomeratum JCM 11608T (98.45, 98.24, 98.12, 98.10 and 98.10 % similarity, respectively), whereas the sequence similarity values with respect to the other Brachybacterium species with validly published names were between 97.4 and 94.2 %. However, the DNA-DNA hybridization values between strain KWS-1T and the five most closely related species were less than the threshold value for species discrimination. The major lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine and the major quinone was menaquinone MK-7. The DNA G+C content of strain KWS-1T was 71.8 mol%. The above data in combination with the phenotypic distinctiveness of strain KWS-1T from other reference strains clearly indicate that the strain represents a novel species, for which the name Brachybacterium aquaticum sp. nov. is proposed. The type strain is KWS-1T (=MTCC 11836T=DSM 28796T=JCM 30059T).

Phenotypic and Genomic Properties of Brachybacterium vulturis sp. nov. and Brachybacterium avium sp. nov.

“…Genes involved in the synthesis of phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylserine (PS), and phosphatidylethanolamine (PE) are not present in the two strains while PE was detected by two-dimensional TLC. This latter identification needs to be confirmed; it is an atypical polar lipid in the majority of Brachybacterium species although PE has been detected in B. aquaticum ( Kaur et al, 2016 ). All nine strains analyzed have genes for the synthesis of DPG and PG.…”

“…The genus Brachybacterium was first described by Collins et al (1988) . At the time of writing, the genus contains 19 species with validly published names: B. faecium ( Collins et al, 1988 ), B. nesterenkovii ( Gvozdyak et al, 1992 ), B. conglomeratum , B. paraconglomeratum , B. rhamnosum ( Takeuchi et al, 1995 ), B. alimentarium , B. tyrofermentans ( Schubert et al, 1996 ), B. fresconis , B. sacelli ( Heyrman et al, 2002 ), B. muris ( Buczolits et al, 2003 ), B. phenoliresistens ( Chou et al, 2007 ), B. zhongshanense ( Zhang et al, 2007 ), B. saurashtrense ( Gontia et al, 2011 ), B. squillarum ( Park et al, 2011 ), B. ginsengisoli ( Hoang et al, 2014 ), B. huguangmaarense ( Liu et al, 2014 ), B. hainanense ( Liu et al, 2015 ), B. aquaticum ( Kaur et al, 2016 ) and B. horti ( Singh et al, 2016 ). The genus Brachybacterium is affiliated to the family Dermabacteraceae with the three genera Dermabacter , Devriesea , and Helcobacillus .…”

Brachybacterium massiliense sp. nov., a new bacterium isolated from stool from a healthy Senegalese child

“…Brachybacterium faecium is the type strain of the genus isolated from poultry deep litter in 1966 [1]. Brachybacteria species have been isolated from a stool sample of a healthy 3-year-old child [2] but also from environmental samples such as garden soil, salt-fermented seafood, oil-contaminated coastal sand, sediment samples and seawater [3], [4], [5].…”

Metagenomics Analysis Reveals an Extraordinary Inner Bacterial Diversity in Anisakids (Nematoda: Anisakidae) L3 Larvae

“…In cluster 2 is represented by the 29 genera recorded in Table 3 . It can be considered as the most important genera: Brachybacterium which has been isolated from sea water [ 113 ]; Dietzia considered as opportunistic human pathogen [ 114 ]; Rothia which causes a wide range of serious infections, especially in immunocompromised hosts [ 115 ]; Mycobacterium , a genus free-living in soil and water widely distributed but the major habitat for some species is the diseased tissue of warm-blooded hosts ( M . tuberculosis and M. leprae are the most known pathogens of this genus for humans) [ 116 ]; some species of Nocardia cause disease in immunocompromised patients [ 117 ].…”