Four novel amicoumacins, namely lipoamicoumacins A–D (1–4), and one new bacilosarcin analog (5) were isolated from culture broth of a marine-derived bacterium Bacillus subtilis, together with six known amicoumacins. Their structures were elucidated on the basis of extensive spectroscopic (2D NNR, IR, CD and MS) analysis and in comparison with data in literature.
A novel Gram-negative, aerobic, catalase-and oxidase-positive, non-sporulating, non-motile, rodshaped bacterium, designated strain UST081027-248 T , was isolated from seawater of the Red Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UST081027-248 T fell within the genus Erythrobacter. Levels of 16S rRNA gene sequence similarity between the novel strain and the type strains of Erythrobacter species ranged from 95.3 % (with Erythrobacter gangjinensis) to 98.2 % (with Erythrobacter citreus). However, levels of DNA-DNA relatedness between strain UST081027-248 T and the type strains of closely related species were below 70 %. Optimal growth of the isolate occurred in the presence of 2.0 % NaCl, at pH 8.0-9.0 and at 28-36 6C. The isolate did not produce bacteriochlorophyll a. The predominant cellular fatty acids were C 17 : 1 v6c, summed feature 8 (C 18 : 1 v6c and/or C 18 : 1 v7c) and C 15 : 0 2-OH. The genomic DNA G+C content of strain UST081027-248 T was 60.4 mol%. Phenotypic properties and phylogenetic distinctiveness clearly indicated that strain
A Gram-negative, catalase-and oxidase-positive, non-sporulating, rod-shaped and slightly halophilic bacterial strain, designated UST090418-1611 T , was isolated from the marine sponge Xestospongia testudinaria collected from the Red Sea coast of Saudi Arabia. Phylogenetic trees based on the 16S rRNA gene sequence placed strain UST090418-1611 T in the family .4 and 97.3 %, respectively), the relatedness of the strain to these three strains in DNA-DNA hybridization was only 58, 56 and 33 %, respectively, supporting the novelty of the strain. In contrast to most strains in the genus Marinobacter, strain UST090418-1611 T tolerated only 6 % (w/v) NaCl, and optimal growth occurred at 2.0 % (w/v) NaCl, pH 7.0-8.0 and 28-36 6C. The predominant cellular fatty acids were C 12 : 0 3-OH, C 16 : 0 , C 12 : 0 and summed feature 3 (C 16 : 1 v6c and/or C 16 : 1 v7c). The genomic DNA G+C content was 57.1 mol%. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we suggest that the strain represents a novel species in the genus Marinobacter, for which the name Marinobacter xestospongiae sp. nov. is proposed, with UST090418-1611 T (5JCM 17469 T 5NRRL B-59512 T ) as the type strain.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.