Cathelicidins, a family of host-defence peptides, are present in a diverse range of species, including fish, amphibians, birds, reptiles, and mammals. The evidence for the role of these cationic antimicrobial peptides in innate host defenses is convincing from the data of animal model and transgenic animal experiments as well as wildlife or domestic animals, indicating that the peptides protect against inflammatory and immune system after induction by bacterial infection. In this study, we present the assessment of transcription expression of CATHL4 gene which encodes indolicidin, a cathelicidin from indigenous yellow cattle of Vietnam. The research focused on RNA samples extracted from lung tissues and lymph node collected from diseased cattle which died of infectious respiratory symptoms and the healthy cattle which were slaughtered for food purposes. By quantitative real-time PCR, the relative expression of transcripts was determined and analyzed using the expression of YWHAZ gene as reference. The results showed that this gene was abundantly expressed at a higher level in tissues of the diseased cattle than in those of the healthy ones. In both infection and healthy states, the expression of CATHL4 in lymph nodes were higher than in lung tissues. This indicated that CATHL4 (indolicidin) may participate in functions against infectious pathogens.
The present study was conducted to evaluate the effects of 6-dimethylaminopurine (6-DMAP), cytochalasin B (CyB) and cycloheximide (CHX) on in vitro development of “I” pig somatic cell nuclear transfer embryos. Oocytes after SCNT were activated with 2 mM 6-DMAP; 7.5 µg/ml CyB and 10 µg/ml CHX. The results indicated that the rate of oocytes no lysis in group activated by CHX was significantly lower compared with those in group activated by 6-DAMP and CyB (90.91% and 88.01%, respectively, P < 0,05). The blastocyst formation rate did not differ between group actvated by 6-DAMP and CyB (24.93% vs 24.4%, P > 0.05). Meanwhile the blastocyst formation rate in group activated by CHX was 15.01%, significantly lower than that in group activated by 6-DAMP and CyB (P < 0.05). The average number of cells in the blastocyst in group activated by 6-DAMP and CyB were higher than that in group activated by CHX (47.78, 44.57 vs 39.42, respectively). The use of 6-DMAP and CyB in the activation of pig oocytes after SCNT was more effective than CHX. The results of this study show that for the first time in Vietnam, we have created “I” pig SCNT embryo without zona pellucida with the rate of blastocyst formation from 15.01% to 24.93%.
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