Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCRquality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major mediating role in orthodontic tooth movement and periodontitis. Despite corresponding in-vitro gene expression studies being a focus of interest for many years, no information is available for hPDL fibroblasts on suitable reference genes, which are generally used in RT-qPCR experiments to normalize variability between samples. The aim of this study was to identify and validate suitable reference genes for normalization in untreated hPDL fibroblasts as well as experiments on orthodontic tooth movement or periodontitis (Aggregatibacter actinomycetemcomitans). We investigated the suitability of 13 candidate reference genes using four different algorithms (geNorm, NormFinder, comparative ΔC q and BestKeeper) and ranked them according to their expression stability. Overall PPIB (peptidylprolyl isomerase A), TBP (TATA-box-binding protein) and RPL22 (ribosomal protein 22) were found to be most stably expressed with two genes in conjunction sufficient for reliable normalization. This study provides an accurate tool for quantitative gene expression analysis in hPDL fibroblasts according to the MIQE guidelines and shows that reference gene reliability is treatment-specific.Orthodontics and periodontology are specialties of dentistry tending to the treatment of misaligned teeth/jaws and bacterially induced inflammation of the periodontal tissues (periodontitis), respectively, with several interactive associations existing 1 . In orthodontics mechanical forces applied to the teeth result in tensile and pressure zones within the periodontal ligament (PDL) 2 . PDL fibroblasts react to this mechanical strain with an increased synthesis of proinflammatory enzymes, cytokines and chemokines 2-4 , triggering osteoclastogenesis. Bacterial toxins from periodontal pathogens in periodontitis, such as the gram-negative Aggregatibacter actinomycetemcomitans (Agac), the key pathogen in aggressive periodontitis 5 , can in a similar way stimulate PDL fibroblasts, which are thus essential both for mediating orthodontic tooth movement and bacterial periodontitis.Real-time quantitative PCR (RT-qPCR) and DNA microarray analysis are the methods of choice to analyse transcription of cellular genes 6,7 . In contrast to microarray analysis, which allows expression profiling of a high number of genes, RT-qPCR enables a precise quantification of gene expression differences in physiological, pathological and various experimental states [8][9][10] . However, a reliable RT-qPCR setup is necessary to achieve valid results. To improve quality and reproducibility of RT-qPCR experiments, Bustin et al. published the MIQE guidelines 11 in 2009, detailing the minimum information for publication of quantitative real-time PCR experiments. A view in current literature shows that m...
Bone is a connective tissue and guarantees protection and support of organ function. Contrary to the common view, bone is a dynamic tissue that constantly undergoes turnover in order to maintain stability and integrity. In this process called bone turnover or bone remodelling, two effector cell types are involved. Osteoclasts, specialised for bone resorption, and osteoblasts, responsible for bone formation, are key players in bone turnover. In the past decade, a lot of information about signal pathways, osteoblast-osteoclast communication and osteoclast activation concerning bone remodelling has arisen. In this publication, we aim to review molecular and biochemical insights with respect to the bone remodelling process. The bone remodelling process is of fundamental importance for craniofacial growth, orthodontic tooth movement and regenerative dentistry.
Aim The aim of the present randomized, controlled trial was to compare the impact of the regular use of a fluoride‐free microcrystalline hydroxyapatite (HAP) dentifrice and a 1400 ppm fluoride control dentifrice on caries progression in 150 highly caries‐active orthodontic patients. Methods The primary outcome was the occurrence of lesions with International Caries Detection and Assessment System (ICDAS) ≥code 1 on the vestibular surfaces of teeth 15‐25 within 168 days after fixation of orthodontic brackets. Secondary outcomes were lesion development ICDAS ≥code 2, the plaque index, and the gingival index. Results In total, 147 patients were included in the intent‐to‐treat (ITT) analysis; 133 finished the study per protocol (PP). An increase in enamel caries ICDAS ≥code 1 was observed in 56.8% (ITT) and 54.7% (PP) of the HAP group participants compared with 60.9% (ITT) and 61.6% (PP) of the fluoride control group. Non‐inferiority testing (ITT and PP) demonstrated the absence of a significant difference between the groups. No significant differences in secondary outcomes were observed between the groups. Conclusion In highly caries‐active patients, the impact of the regular use of a microcrystalline HAP dentifrice on caries progression is not significantly different from the use of a 1400 ppm fluoride toothpaste (ClinicalTrials.gov: NCT02705456).
hPDL fibroblasts seem to play a major mediating role in the early phase of OTM with a differentiated, time-dependent regulation and expression pattern of cytokines and other mediators.
This retrospective study examined the occurrence of congenitally missing permanent teeth and the need for dental treatment in the Regensburg University Medical Centre of Eastern Bavaria. Using a dental administration software tool, a total of 1442 patients who presented for orthodontic treatment between 1994 and 2006 were identified. After exclusion of 89 patients with incomplete records, 1353 subjects (635 males and 718 females) remained for analysis. Of these, 1130 had no missing permanent teeth, 52 had cleft lips, 110 had one to two teeth missing, 34 had three to five missing teeth, and 27 had greater than or equal to six missing teeth. The analyses focused on the type and number of missing teeth and on differences in the severity of dental agenesis according to gender and to referrals from various geographic regions around Regensburg. The data were statistically analysed using two-tailed tests. The following teeth were most frequently missing: tooth 35 (5.9 per cent), 45 (5.1 per cent), 22 (4.0 per cent), 12 (3.6 per cent), 15 (3.1 per cent), and 25 (3.0 per cent). No statistically significant difference in gender was found for one to two missing permanent teeth (low degree), hypo- or oligodontia (severe degree), or cleft lip. The odds ratio (OR) of presenting with hypo- or oligodontia compared with no missing teeth was higher among subjects originating from geographic regions outside Regensburg than from those from Regensburg, and it was statistically significantly higher for patients from Passau {OR = 3.53 [95% confidence interval (CI) = 1.18-10.52]} and Landshut [OR = 3.65 (95% CI = 1.22-10.99)]. The high prevalence and severe degree of dental agenesis of permanent teeth found in these groups of patients likely reflects distinct referral patterns for patients originating from geographic regions outside Regensburg. These data reinforce the need for a specialized dental treatment centre with the capacity to adequately serve a large rural area in Eastern Bavaria.
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