Expression kinetics of human periodontal ligament fibroblasts in the early phases of orthodontic tooth movement

Schröder, Agnes; Bauer, K. H.; Spanier, Gerrit; Proff, Peter; Wolf, Michael; Kirschneck, Christian

doi:10.1007/s00056-018-0145-1

Cited by 63 publications

(122 citation statements)

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“…It plays a major role in cell adhesion, growth, migration, and differentiation , as well as in tissue repair, and connects collagen fibers. It has been reported to be associated with extracellular matrix remodeling during orthodontic tooth movement , but in line with other data, pressure application had no impact on expression of FN1 . It has been reported, in a different context, that NaCl has an effect on extracellular matrix reorganization, as Na + accumulation in the skin led to enhanced production of glycosaminoglycans (GAGs) and upregulation of sulfation of GAGs .…”

Section: Discussionsupporting

confidence: 59%

“…Then, 2 ml of DMEM per well was added and, after a 24‐h preincubation phase, cells were incubated for another 48 h with addition of either 0 mM NaCl (control) or 40 mM NaCl (Sigma‐Aldrich), with or without compressive orthodontic force application of 2 g cm −2 . The latter was exerted by a sterile glass disc of defined size and weight according to a well‐established and published method (Fig. ).…”

Section: Methodsmentioning

confidence: 99%

“…Periodontal ligament fibroblasts are known to react to orthodontic compressive forces by triggering a local sterile pseudoinflammatory immunological process within the periodontal ligament, thus enabling orthodontic tooth movement via increased release of prostaglandins synthesized by enhanced expression and activity of cyclooxygenase‐2 (COX‐2) . This leads to enhanced secretion of proinflammatory cytokines, such as interleukin (IL)‐6, as well as the formation of soluble and membrane‐linked receptor activator of nuclear factor kappa‐B ligand (RANKL) and osteoclastogenesis . Furthermore, mechanically stimulated PDLFs secrete matrix metalloproteinases , which are responsible for degradation of the extracellular matrix , thus inducing the necessary transformation for orthodontic tooth movement.…”

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confidence: 99%

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Sodium‐chloride‐induced effects on the expression profile of human periodontal ligament fibroblasts with focus on simulated orthodontic tooth movement

Schröder¹,

Nazet²,

Neubert

et al. 2019

European J Oral Sciences

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Increased salt (NaCl) consumption triggers chronic diseases such as hypertension or osteopenia. Its impact on orthodontic tooth movement and periodontitis, however, has not been investigated, although both processes are related to the immune system, with periodontal ligament fibroblasts (PDLFs) playing a key mediating role. Here, we investigated the impact of NaCl on the expression pattern of PDLFs in a model of simulated compressive orthodontic strain. Periodontal ligament fibroblasts were preincubated for 24 h with additional 0 or 40 mM NaCl and concurrently treated for another 48 h with or without compressive strain of 2 g cm−2. We analyzed the expression of genes and proteins involved in orthodontic tooth movement by reverse transcription quantitative polymerase chain reaction (RT‐qPCR), ELISA, and immunoblot. Co‐culture experiments were performed to observe PDLF‐mediated osteoclastogenesis. A higher (40 mM) concentration of NaCl in the culture medium resulted in increased secretion of prostaglandin, expression of alkaline phosphatase, and expression of genes involved in extracellular matrix remodeling, but decreased compression‐induced expression of the interleukin‐6 (IL6) gene. The 40 mM concentration of NaCl also enhanced receptor activator of nuclear factor kappa‐B ligand (RANKL) but reduced that of osteoprotegerin (OPG), resulting in upregulated PDLF‐mediated osteoclastogenesis. A high NaCl concentration in the periodontal ligament, corresponding to a high‐salt diet in vivo, may influence orthodontic tooth movement and periodontitis through increased secretion of prostaglandins by PDLFs and upregulated PDLF‐mediated osteoclastogenesis, possibly accelerating orthodontic tooth movement and propagating periodontitis and periodontal bone loss.

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Section: Discussionsupporting

confidence: 59%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Sodium‐chloride‐induced effects on the expression profile of human periodontal ligament fibroblasts with focus on simulated orthodontic tooth movement

Schröder¹,

Nazet²,

Neubert

et al. 2019

European J Oral Sciences

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“…As a result, a sterile inflammatory reaction occurs in the periodontal ligament, which is mainly mediated by periodontal ligament fibroblasts (PDLF) [2,3], but also involves cells of the immune system such as macrophages, lymphocytes and T cells [4,5]. Stimulation by mechanical forces induces secretion of proinflammatory enzymes, cytokines and chemokines by PDLF [3,6,7]. Furthermore PDLF enhance receptor activator of NF-kB ligand (RANKL) expression and reduce osteoprotegerin secretion upon pressure application [3,6,8], thus promoting differentiation of osteoclast progenitor cells to bone-resorptive osteoclasts [7,9].…”

Section: Introductionmentioning

confidence: 99%

“…Stimulation by mechanical forces induces secretion of proinflammatory enzymes, cytokines and chemokines by PDLF [3,6,7]. Furthermore PDLF enhance receptor activator of NF-kB ligand (RANKL) expression and reduce osteoprotegerin secretion upon pressure application [3,6,8], thus promoting differentiation of osteoclast progenitor cells to bone-resorptive osteoclasts [7,9]. According to the biphasic theory of orthodontic tooth movement (OTM), movement is achieved via mechanically stressed PDLF-and lymphocyte-regulated bone resorption processes with subsequent bone formation via osteoclast-stimulated osteoblasts with numerous cell-cell interactions [2,3,10].…”

Section: Introductionmentioning

confidence: 99%

Effects of histamine on human periodontal ligament fibroblasts under simulated orthodontic pressure

et al. 2020

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As type-I-allergies show an increasing prevalence in the general populace, orthodontic patients may also be affected by histamine release during treatment. Human periodontal ligament fibroblasts (PDLF) are regulators of orthodontic tooth movement. However, the impact of histamine on PDLF in this regard is unknown. Therefore PDLF were incubated without or with an orthodontic compressive force of 2g/cm 2 with and without additional histamine. To assess the role of histamine-1-receptor (H1R) H1R-antagonist cetirizine was used. Expression of histamine receptors and important mediators of orthodontic tooth movement were investigated. PDLF expressed histamine receptors H1R, H2R and H4R, but not H3R. Histamine increased the expression of H1R, H2R and H4R as well as of interleukin-6, cyclooxygenase-2, and prostaglandin-E2 secretion even without pressure application and induced receptor activator of NF-kB ligand (RANKL) protein expression with unchanged osteoprotegerin secretion. These effects were not observed in presence of H1R antagonist cetirizine. By expressing histamine receptors, PDLF seem to be able to respond to fluctuating histamine levels in the periodontal tissue. Increased histamine concentration was associated with enhanced expression of proinflammatory mediators and RANKL, suggesting an inductive effect of histamine on PDLF-mediated osteoclastogenesis and orthodontic tooth movement. Since cetirizine inhibited these effects, they seem to be mainly mediated via histamine receptor H1R.

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Regulatory mechanisms and regeneration strategies of the soft–hard tissue interface in the human periodontium

Dang,

Wei,

Wan

et al. 2024

BMEMat

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The soft‐hard tissue interface of the human periodontium is responsible for periodontal homeostasis and is essential for normal oral activities. This soft‐hard tissue interface is formed by the direct insertion of fibrous ligaments into the bone tissue. It differs from the unique four‐layer structure of the fibrocartilage interface. This interface is formed by a combination of physical, chemical, and biological factors. The physiological functions of this interface are regulated by different signaling pathways. The unique structure of this soft‐hard tissue interface has inspired scientists to construct biomimetic gradient structures. These biomimetic systems include nanofiber scaffolds, cell sheets, and hydrogels. Exploring methods to repair this soft‐hard tissue interface can help solve clinically unresolved problems. The present review examines the structure of the soft‐hard tissue interface of the periodontium and the factors that influence the development of this interface. Relevant regulatory pathways and biomimetic reconstruction methods are also presented to provide ideas for future research on interfacial tissue engineering.

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Expression kinetics of human periodontal ligament fibroblasts in the early phases of orthodontic tooth movement

Abstract: hPDL fibroblasts seem to play a major mediating role in the early phase of OTM with a differentiated, time-dependent regulation and expression pattern of cytokines and other mediators.

Cited by 63 publications

References 47 publications

Sodium‐chloride‐induced effects on the expression profile of human periodontal ligament fibroblasts with focus on simulated orthodontic tooth movement

Sodium‐chloride‐induced effects on the expression profile of human periodontal ligament fibroblasts with focus on simulated orthodontic tooth movement

Effects of histamine on human periodontal ligament fibroblasts under simulated orthodontic pressure

Regulatory mechanisms and regeneration strategies of the soft–hard tissue interface in the human periodontium

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