The need for high throughput label-free multiplexed sensors for biological sensing has increased in the last decade. In this paper, a new surface stress-based polydimethylsiloxane (PDMS) membrane biosensor for cellular detection is designed and fabricated. The geometric parameters of the PDMS membrane are optimized through the finite element (FE) analysis. One fiber optic interferometer biosensor test system is built to test the characteristics of the biosensor. The biosensor is further functionalized using 11 Mercapto 1 undecanoicacid (MUA: SH-(CH2)10-COOH) and tested in contact with cells Escherichia coli (E. coli). The results of our experiments showed that cells can be detected based on the surface stress-based PDMS membrane biosensor. The new approach for cellular detection has good sensitivity and biocompatibility.
Residue of alloy anode generated in three-layer liquid electrolytic purifying method is refined, the state of iron in raw materials is analysed. The state (morphology) changes of iron refined by different refining agent are investigated. Results show: 40% of boron salts or phosphate as the main component of refining agent adding 15% NaCl、20% KCl、15% CaF2、5% NaF、5% cryolite, melting the residue of alloy anode at 1100°C and 1200°C respectively, the crystal could be incubated to grow up fully, it is benefit for collecting iron. When the soaking time is 150min, iron component in alloy is 4.8%,it reaches the standard taking back into the aluminum refining process.
Based on magnetoelastic biosensor for the heavy ion detection, this paper is committed to study the influence of the excitations and detection positions on the sensitivity of magnetoelastic biosensors. The frequency response of biosensors with different excitations and different detection positions were studied. The numerical simulation software ANSYS [TM] Ansoft Maxwell was applied to calculate the distribution of magnetic field around the coil. The optimal excitation value was determined by this simulation. The frequency test of the magnetoelastic sensor was analyzed by a network analyzer, which showed the frequency in the middle of coil is better than in the two ends.
Surface stress-based biosensors as a crucial part of micro-scale and label-free system, use free energy change, the underlying concept in any binding reaction, have been investigated extensively in recent years. In this paper, a new bi-micro-cantilever surface stress biosensor is proposed which can be used to detect cells. Some fundamental study has been done, especially for the micro-cantilever due to its crucial role in the whole system. To acquiring the optimal material for more sensitive sensor, four material, Si, SiN, AlN, PMMA(polymethylmethacrylate), were contrastively analyzed under the same conditions (loads, size, environmental factor. etc) by finite element (FE) method. This study could provide some foundation for the biosensor design and fabrication.
Here we demonstrate a microfluidic-based analysis system based on single cell capture array, which can physically trap individual cell using micrometer-sized structures. A stable and in vivo-like microenvironment was built with the novel structure at the single-cell detection level. The microfluidic-based design can decouple single cells from fluid flow with the help of micropillars. The size and geometry of the cell jails are designed in order to discriminate between mother and daughter cells. It provides an experimental platform to efficiently monitor individual cell state for a long period of time. Furthermore, the parallel microfluidic array can ensure accuracy. In addition, finite element method (FEM) was employed to predict fluid transport properties for the most optimal fluid microenvironment.
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