Surface stress-based biosensors as a crucial part of micro-scale and label-free system, use free energy change, the underlying concept in any binding reaction, have been investigated extensively in recent years. In this paper, a new bi-micro-cantilever surface stress biosensor is proposed which can be used to detect cells. Some fundamental study has been done, especially for the micro-cantilever due to its crucial role in the whole system. To acquiring the optimal material for more sensitive sensor, four material, Si, SiN, AlN, PMMA(polymethylmethacrylate), were contrastively analyzed under the same conditions (loads, size, environmental factor. etc) by finite element (FE) method. This study could provide some foundation for the biosensor design and fabrication.
Here we demonstrate a microfluidic-based analysis system based on single cell capture array, which can physically trap individual cell using micrometer-sized structures. A stable and in vivo-like microenvironment was built with the novel structure at the single-cell detection level. The microfluidic-based design can decouple single cells from fluid flow with the help of micropillars. The size and geometry of the cell jails are designed in order to discriminate between mother and daughter cells. It provides an experimental platform to efficiently monitor individual cell state for a long period of time. Furthermore, the parallel microfluidic array can ensure accuracy. In addition, finite element method (FEM) was employed to predict fluid transport properties for the most optimal fluid microenvironment.
Cellular culture is a complex process for cells are grown under certain given conditions, generally not in vivo. In this paper, a novel microfluidic was designed for cellular culture in a long term. Its structure mainly consists of two parts: channels and chambers. In this architecture, two kinds of channels are designed. One is used to load cell into chambers, the other is for the load of culture solution and drugs. Due to diffusion effect, the culture solution and drugs can permeate into chambers through a amount of ostioles. Finite element simulation was utilized to demonstrast the velocity field distribution and concentration field distribution. Comparisons were made to verify the rationality of the design. The simulation results suggest that this novel microfluidic is appropriate suitable in terms of culturing cells.
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