Acute altitude hypoxia represents the cause of multiple adverse consequences. Current treatments are limited by side effects. Recent studies have shown the protective effects of resveratrol (RSV), but the mechanism remains unknown. To address this, the effects of RSV on the structure and function of hemoglobin of adult (HbA) were preliminarily analyzed using surface plasmon resonance (SPR) and oxygen dissociation assays (ODA). Molecular docking was conducted to specifically analyze the binding regions between RSV and HbA. The thermal stability was characterized to further validate the authenticity and effect of binding. Changes in the oxygen supply efficiency of HbA and rat RBCs incubated with RSV were detected ex vivo. The effect of RSV on the anti-hypoxic capacity under acute hypoxic conditions in vivo was evaluated. We found that RSV binds to the heme region of HbA following a concentration gradient and affects the structural stability and rate of oxygen release of HbA. RSV enhances the oxygen supply efficiency of HbA and rat RBCs ex vivo. RSV prolongs the tolerance times of mice suffering from acute asphyxia. By enhancing the oxygen supply efficiency, it alleviates the detrimental effects of acute severe hypoxia. In conclusion, RSV binds to HbA and regulates its conformation, which enhances oxygen supply efficiency and improves adaption to acute severe hypoxia.
Adequate oxygen is identified as one of major factors in the processes of wound healing. Various strategies of oxygen therapy have been developed to improve wound healing, such as oxygen carriers. Herein polydopamine coated hemoglobin (Hb-PDA) nanoparticles as one of Hemoglobin-based Oxygen Carriers (HBOCs) for oxygen supply is proposed and synthesized, Hb-PDA nanoparticles accelerate the wound repair process in an excisional full-thickness mouse model. And inflammatory phased of wounds can be shortened by antioxidant properties of polydopamine (PDA) modification. Moreover, Hb-PDA nanoparticles efficiently promote the formation of blood vessel by upregulating expression of vascular endothelial growth factor (VEGF) and angiogenesis related protein. Interestingly, Hb-PDA nanoparticles improve the oxygen supply of wounds in the wound area, as well as down-regulated expression of Hypoxia-inducible factor-1α (HIF-1α). The results indicate the Hb-PDA nanoparticles might be a promising approach for efficient wound healing with the capacity to improve oxygen supply.
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