Inflammatory bowel disease including ulcerative colitis are complex multifactorial diseases of unknown aetiology. Sulphate-reducing bacteria are often associated with the occurrence of the disease. The physiological properties of intestinal sulphate-reducing bacteria including kinetic characteristic of their growth have never been reported. The aim of this research was to evaluate the presence of sulphate-reducing bacteria isolated from the intestines of mice, study their growth, calculate and compare the kinetic growth properties on the model of dextran sulphate sodium induced ulcerative colitis in the mice. The number of viable intestinal sulphate-reducing bacteria from the bowel lumen of mice with ulcerative colitis was higher (P > 0.05) by 22% at 12 h of cultivation compared with cultures of sulphate-reducing bacteria from the bowel lumen of healthy mice. The sulphate-reducing bacteria from mice with colitis also had a slightly higher generation time (14.29 h) and exponential growth phase (22.24 h) compared with cultures from healthy mice. The time of lag-phase was 2 × shorter (P > 0.01) in the cultures of sulphate-reducing bacteria from mice with ulcerative colitis. The described research is new and important for the prediction of the sulphate-reducing bacteria number in the gut and their rate of dissimilatory sulphate reduction. The kinetic characteristic of their growth is important for further clarification of the mechanisms of sulphate reduction and accumulation of hydrogen sulphide, which is toxic for epithelial cells of the intestine and can cause bowel diseases both in humans and animals, in particular ulcerative colitis. Intestinal microbiota, growth rate, hydrogen sulphide, bowel diseasesSulphate-reducing bacteria (SRB) are widespread in anaerobic areas of soils, wetlands, fresh and marine waters and available in the microbiota of the large intestine of humans and animals (Barton and Hamilton 2010). These microorganisms metabolize sulphate as an electron acceptor to hydrogen sulphide. The sulphate dissimilation process is called the "dissimilatory sulphate reduction" or "sulphate respiration" (Kushkevych 2016a,b). For this process, SRB needs exogenous electron donors, including organic compounds or molecular hydrogen. Dependent on SRB genera, organic compounds are oxidized incompletely to acetate (acetogenic SRB) or completely to carbon (IV) oxide (Barton and Hamilton 2010).The intensity of sulphate reduction in SRB and, accordingly, the accumulation of hydrogen sulphide in high or toxic concentrations in the intestines can lead to the development of various diseases (Kushkevych 2014a). Hydrogen sulphide is the final product in the sulphate reduction process of SRB metabolism. At high concentrations, this final metabolite
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.