Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis

Abstract

Inflammatory bowel disease including ulcerative colitis are complex multifactorial diseases of unknown aetiology. Sulphate-reducing bacteria are often associated with the occurrence of the disease. The physiological properties of intestinal sulphate-reducing bacteria including kinetic characteristic of their growth have never been reported. The aim of this research was to evaluate the presence of sulphate-reducing bacteria isolated from the intestines of mice, study their growth, calculate and compare the kinetic growth properties on the model of dextran sulphate sodium induced ulcerative colitis in the mice. The number of viable intestinal sulphate-reducing bacteria from the bowel lumen of mice with ulcerative colitis was higher (P > 0.05) by 22% at 12 h of cultivation compared with cultures of sulphate-reducing bacteria from the bowel lumen of healthy mice. The sulphate-reducing bacteria from mice with colitis also had a slightly higher generation time (14.29 h) and exponential growth phase (22.24 h) compared with cultures from healthy mice. The time of lag-phase was 2 × shorter (P > 0.01) in the cultures of sulphate-reducing bacteria from mice with ulcerative colitis. The described research is new and important for the prediction of the sulphate-reducing bacteria number in the gut and their rate of dissimilatory sulphate reduction. The kinetic characteristic of their growth is important for further clarification of the mechanisms of sulphate reduction and accumulation of hydrogen sulphide, which is toxic for epithelial cells of the intestine and can cause bowel diseases both in humans and animals, in particular ulcerative colitis. Intestinal microbiota, growth rate, hydrogen sulphide, bowel diseasesSulphate-reducing bacteria (SRB) are widespread in anaerobic areas of soils, wetlands, fresh and marine waters and available in the microbiota of the large intestine of humans and animals (Barton and Hamilton 2010). These microorganisms metabolize sulphate as an electron acceptor to hydrogen sulphide. The sulphate dissimilation process is called the "dissimilatory sulphate reduction" or "sulphate respiration" (Kushkevych 2016a,b). For this process, SRB needs exogenous electron donors, including organic compounds or molecular hydrogen. Dependent on SRB genera, organic compounds are oxidized incompletely to acetate (acetogenic SRB) or completely to carbon (IV) oxide (Barton and Hamilton 2010).The intensity of sulphate reduction in SRB and, accordingly, the accumulation of hydrogen sulphide in high or toxic concentrations in the intestines can lead to the development of various diseases (Kushkevych 2014a). Hydrogen sulphide is the final product in the sulphate reduction process of SRB metabolism. At high concentrations, this final metabolite

Recent Advances in Metabolic Pathways of Sulfate Reduction in Intestinal Bacteria

“…The reason for the occurrence of ulcerative colitis and IBD has not been fully revealed yet. Factors, such as dietary habits and intestinal microbiome composition, influence IBD prevalence [26]. A connection between the SRB intestinal occurrence and IBD has been found.…”

“…Only a special group of organisms can extract energy from DSR, while the ASR process is abundant among other organisms (plants and bacteria). SRB are an important group of microorganisms in various ecosystems, including the intestinal tract, because they require inorganic sulfate as an electron acceptor to obtain energy from organic compounds [25,26]. SRB are a significant part of the environment because they require inorganic sulfate for energy production [18].…”

Estuary Sediment Treatment for Reducing Sulfate in Acid Mine Water

“…However, on the 15 th day of treatments T1 and T2 saw a sharp increase in the number of sulfatereducing bacteria up to the 30 th day. This is called the log phase, where the bacterial cells that are able to survive low pH will increase in number (Kushkevych et al, 2017). On the other hand, the sulfate-reducing bacteria in treatment T3 saw almost no increase; this was also the case with treatment T4 as the control treatment where there was even a reduction in the number of cells.…”

“…On the other hand, the sulfate-reducing bacteria in treatment T3 saw almost no increase; this was also the case with treatment T4 as the control treatment where there was even a reduction in the number of cells. This is called the stationary phase and is followed by the death phase that is caused by an unsupportive environment, such as a very acidic environment, low pH, exhausted nutrients or toxic substances produced by the microbes themselves that can inhibit their growth (Meier et al, 2012;Kushkevych et al, 2017).…”

Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria

“…There is an overproduction of hydrogen sulfide in patients suffering from ulcerative colitis. Many research papers show evidence that higher occurrence of SRB and their hydrogen sulfide overproduction is one of the factors in ulcerative colitis [13][14][15]. The prevailing intestinal SRB belong mainly to five genera, namely Desulfovibrio, Desulfobacter, Desulfomonas, Desulfobulbus, and Desulfotomaculum [16][17][18][19].…”