A necessary link in the life of any cell is lipid peroxidation. This process underlies the renewal and restructuring of biological membranes, regulation of their composition, permeability and activity of membrane-bound enzymes. The increase in the level of POL (peroxidation of lipids) leads to oxidative stress, which provokes the formation of various pathological conditions in the body. One of the main components of the antioxidant protection of the body is a group of metal enzymes - superoxide dismutase (SOD), catalyzing the dismutation reaction of superoxide anion radicals and maintaining the concentration of these radicals in the cell at a low level. The mechanism of SOD functioning includes sequential renewal and oxidation of metal ions of variable valence in the active center of the enzyme. SOD is an endogenous acceptor of free oxygen radicals, the excessive accumulation of which in the cell is important in the development of a number of oxygen-dependent pathological processes (hypoxia, inflammation, intoxication, etc.). SOD removes superoxide radicals and prevents the formation of other, more dangerous for the body free radicals: hydroxyl radical and singlet oxygen. The activity of superoxide dismutase in blood and tissues is a marker of metabolic disorders. During the study of the relationship of the distribution of catalase activity in serum and tissues of rats, the following tasks were solved: the activity of SOD in serum and tissues of the liver, brain, heart, as well as in skeletal muscle tissues of rats was indicated; the relationship of the distribution of SOD activity in serum and tissues of rats was revealed. The paper presents the results of nonparametric correlation analysis to assess the relationship of the distribution of SOD activity in serum and tissues of small experimental animals.
In the process of physiological activity of the body, a wide range of toxic metabolites is formed, in particular, hydrogen peroxide, superoxide and hydroxyl radical, which have a destructive effect on cells and cell membranes. One of the natural body protection mechanisms is the enzyme catalase, which accelerates the decomposition of constantly formed hydrogen peroxide to the final products, while oxidizing low molecular alcohols and nitrites, involved in the process of cellular respiration and does not require energy for activation. Catalase activity in blood and tissues is a marker of metabolic disorders. Catalase is localized mainly in the peroxisomes and cytoplasm of the cell, saturates the erythrocytes, liver and kidneys. Catalase activity allows assessing the level of endotoxicosis in the body. In order to study the relationship of the distribution of catalase concentration in serum and rat tissues, the following tasks were solved: the concentration of catalase in serum and tissues of the liver, brain, heart, as well as in skeletal muscle tissues of rats was determined; the correlation of catalase concentration distribution in blood serum and tissues of rat was revealed. The paper presents the results of nonparametric correlation analysis to assess the relationship of catalase activity distribution in serum and tissues of small experimental animals.
Hemato-ophthalmic barrier is one of the mechanisms of body resistance. One of the complications of mechanical trauma of the eye and violation of the hemato-ophthalmic barrier is the emergence of oxidative stress on the background of the general inflammatory process. Normally, oxidative stress in the skeletal muscle tissue is not a damaging agent, but when intensified by other factors, it promotes pathological changes in the body. Objective: to study the dynamics of superoxiddismutase (SOD) activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on the hemato-ophthalmic barrier. Materials and methods: The study was carried out on pedigree matured male rats in the amount of 150 pieces. The activity of SOD in skeletal muscle tissue was studied before the experiment, as well as on the 1st, 3rd, 5th, 7th and 14th day of the experiment using the standard technique of V.S. Gurevich. The obtained digital material was subjected to statistical processing by means of non-parametric statistical analysis. Conclusion: SOD activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on hemato-ophthalmic barrier is most effectively stabilized in standard therapy of mechanical eye injury with the addition of quercetin in the form of injections.
It is known that the majority of pathological processes take place against the background of formation of active oxygen species and intensification of free radical oxidation of bio-substrates. In response to this, the antioxidant system of the cell is activated, and the glutathione system is an important link in this system. The latter can take part in the maintenance of the optimal state of biomembranes, in the processes of detoxification, antioxidant protection, etc. The biological role of glutathione reductase is to maintain high intracellular concentration of reduced glutathione. The aim of our study was to study the relationship between the distribution of glutathione reductase activity in blood serum and rat tissues. In order to achieve the goal of the study the following tasks were solved: the activity of glutathione reductase in blood serum and tissues of liver, brain, heart, as well as in skeletal muscle tissues of rats was determined; the interrelation of the activity distribution of glutathione reductase in blood serum and tissues of rats was revealed. The article presents the results of nonparametric correlation analysis to assess the relationship between the distribution of glutathione reductase activity in blood serum and tissues of small experimental animals.
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