A necessary link in the life of any cell is lipid peroxidation. This process underlies the renewal and restructuring of biological membranes, regulation of their composition, permeability and activity of membrane-bound enzymes. The increase in the level of POL (peroxidation of lipids) leads to oxidative stress, which provokes the formation of various pathological conditions in the body. One of the main components of the antioxidant protection of the body is a group of metal enzymes - superoxide dismutase (SOD), catalyzing the dismutation reaction of superoxide anion radicals and maintaining the concentration of these radicals in the cell at a low level. The mechanism of SOD functioning includes sequential renewal and oxidation of metal ions of variable valence in the active center of the enzyme. SOD is an endogenous acceptor of free oxygen radicals, the excessive accumulation of which in the cell is important in the development of a number of oxygen-dependent pathological processes (hypoxia, inflammation, intoxication, etc.). SOD removes superoxide radicals and prevents the formation of other, more dangerous for the body free radicals: hydroxyl radical and singlet oxygen. The activity of superoxide dismutase in blood and tissues is a marker of metabolic disorders. During the study of the relationship of the distribution of catalase activity in serum and tissues of rats, the following tasks were solved: the activity of SOD in serum and tissues of the liver, brain, heart, as well as in skeletal muscle tissues of rats was indicated; the relationship of the distribution of SOD activity in serum and tissues of rats was revealed. The paper presents the results of nonparametric correlation analysis to assess the relationship of the distribution of SOD activity in serum and tissues of small experimental animals.
Hemato-ophthalmic barrier is one of the mechanisms of body resistance. One of the complications of mechanical trauma of the eye and violation of the hemato-ophthalmic barrier is the emergence of oxidative stress on the background of the general inflammatory process. Normally, oxidative stress in the skeletal muscle tissue is not a damaging agent, but when intensified by other factors, it promotes pathological changes in the body. Objective: to study the dynamics of superoxiddismutase (SOD) activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on the hemato-ophthalmic barrier. Materials and methods: The study was carried out on pedigree matured male rats in the amount of 150 pieces. The activity of SOD in skeletal muscle tissue was studied before the experiment, as well as on the 1st, 3rd, 5th, 7th and 14th day of the experiment using the standard technique of V.S. Gurevich. The obtained digital material was subjected to statistical processing by means of non-parametric statistical analysis. Conclusion: SOD activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on hemato-ophthalmic barrier is most effectively stabilized in standard therapy of mechanical eye injury with the addition of quercetin in the form of injections.
It is known that the majority of pathological processes take place against the background of formation of active oxygen species and intensification of free radical oxidation of bio-substrates. In response to this, the antioxidant system of the cell is activated, and the glutathione system is an important link in this system. The latter can take part in the maintenance of the optimal state of biomembranes, in the processes of detoxification, antioxidant protection, etc. The biological role of glutathione reductase is to maintain high intracellular concentration of reduced glutathione. The aim of our study was to study the relationship between the distribution of glutathione reductase activity in blood serum and rat tissues. In order to achieve the goal of the study the following tasks were solved: the activity of glutathione reductase in blood serum and tissues of liver, brain, heart, as well as in skeletal muscle tissues of rats was determined; the interrelation of the activity distribution of glutathione reductase in blood serum and tissues of rats was revealed. The article presents the results of nonparametric correlation analysis to assess the relationship between the distribution of glutathione reductase activity in blood serum and tissues of small experimental animals.
The influence of biologically active compounds on the functional state of the central nervous system and the emotional component in rats can be evaluated by using behavioral methods, which are based on studying the exploratory behavior of animals in an environment new to them. One such method is the study of animal behavior in an elevated cross-shaped maze with open and closed arms and the Porsolt "despair" test. The aim of our study was to investigate the emotional state of the offspring of rats treated with aqueous plant extracts of Centella asiatica, Ginkgo biloba, Eleuterococcus and a mixture of aqueous extracts of Centella asiatica and Ginkgo biloba. The study of the emotional state of the offspring of rats treated with aqueous plant extracts was performed on 3 month old animals obtained in the experiment from males (20 animals) and females (50 animals), which received aqueous plant extracts at a dose of 30 mg/100 g of the animal body weight, in the volume of 1 ml for 30 days. Conclusions: Aqueous extracts of Centella asiatica, Ginkgo biloba and Eleutherococcus have pronounced nootropic activity, as the evaluation of animal anxiety level in the Elevated Cross Maze test and depression level in the Porsolt Despair Behavior test show a significant difference in the behavior of intact rats and offspring rats, receiving aqueous plant extracts as an additional load and the most pronounced effect is observed with the complex application of extracts of Centella asiatica and Ginkgo biloba.
Chronic recurrent aphthous stomatitis (CRAS) is a disease of the oral mucosa, characterized by the appearance of specific round aphthous forms with hyperemic corolla, covered with grayish-yellow plaque against the background of inflamed mucous membrane, which violates the oxidative homeostasis of oral fluid and the body as a whole. The aim of our study was to reveal the dynamics of free radical processes in oral fluid of patients with chronic recurrent aphthous stomatitis on the background of the first stage of antiseptic therapy with analgesics in order to determine the optimal treatment tactics for this condition. In order to achieve the set objective we had to solve the following tasks: to evaluate the functional activity of free radical processes in the oral liquid of patients with CPAS on the background of antiseptics (chlorhexidine, octenisept, furacilin, myramistine) and anaesthetic (Camistad gel) application for 7 days. Materials and Methods. During the study the oral fluid of 120 people diagnosed with CPPS was studied, divided into 4 groups equally: Group 1 – control, patients used the antiseptic chlorhexidine for gargling; Group 2 – patients with CPPS therapy with octenisept; Group 3 – patients with CPPS therapy with furacilin; Group 4 – patients with CPPS therapy with miramistine. For pain relief, patients in all groups used Kamistad gel. Analysis of free radical processes in oral fluid of patients was carried out before the study and on the 7th day of therapy by determining products of oxidative modification of biomolecules on the basis of quantitative evaluation of stained complex with thiobarbituric acid (TBA), maximal flash (MVHL) and area (PCL) of H2O2-induced luminol-dependent chemiluminescence using LT-01 chemiluminometer ("Horos" "Joint Venture Soviet-Swedish Company"). Concentration of diene conjugates and malonic dialdehyde was also determined to estimate enzymatic part of oxidative homeostasis. Results of the study. Results of the researches permit to conclude that at chronic recurrent aphthous stomatitis enzymatic activity of an oral liquid changes. According to the presented data the initial values of concentration of TBK-RP, maximal flash of oral chemiluminescence, area of oral chemiluminescence and concentration of MDA and DC in the control group and three experimental groups reliably differ from the data received on the 7th day of the research. Significant decrease of the studied indexes of oral liquid testifies to the efficiency of the first stage of therapy of aphthous stomatitis by antiseptic preparations and decrease of intensity of oxidative processes in oral liquid. Of all the proposed antiseptics the best efficiency is demonstrated by octenisept, reliably worse are chlorhexidine and myramistine and the least effective in the therapy of CPAS is furacilin. Conclusions: treatment of CPAS with Octenisept in combination with Camistad gel is the most effective in restoration of the disturbed oxilite
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