People suffering from food allergies are dependent on accurate food labeling, as an avoidance diet is the only effective countermeasure. Even a small amount of allergenic protein can trigger severe reactions in highly sensitized patients. Therefore, sensitive and reliable tests are needed to detect potential cross-contamination. In this paper two fast sandwich immunoassays are described for the determination of peanut (Arachis hypogaea) and hazelnut (Corylus avellana) traces in complex food matrices. Mouse monoclonal antibodies were used as capture antibodies, and labeled rabbit polyclonal antibodies were used as detection antibodies in both assays. The assay time was 30 min in total, and cross-reactivities against a variety of fruits and seeds were found to be in the low 10(-4)% (ppm) level or in some cases not detectable. The recoveries in all tested food matrices ranged from 86 to 127%, and the limits of detection were in the range of 0.2-1.2 mg/kg (ppm) in food for both peanut and hazelnut, respectively.
It is known that formaldehyde is produced by the breakdown of trimethy1amine-Noxide (TMAO) by enzyme action during frozen storage of gadoid muscle. However, little is known of the nature of the enzyme's activity during frozen storage of the fish muscle. Determination of the formaldehyde produced throughout the depth of a block of minced cod muscle during frozen storage has shown that the production of formaldehyde is inhibited near the surface of the mince. Both in-vivo and in-vitro studies using TMAO as substrate have shown that the production of formaldehyde is inhibited by both oxygen and potassium cyanide, and activated by reduced nicotinamide dinucleotide.
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