“…The use of real-time PCR technique, largely used for the detection and quantiWcation of genetically modiWed organisms (GMOs, Pardigol, Guillet, & Popping, 2003;Ronning, Vaitilingom, Berdal, & Holst-Jensen, 2003) and recently suggested for the peanut detection in foods (Hird, Lloyd, Goodier, Brown, & Reece, 2003), allows rapid and sensitive detection of a speciWc genomic DNA sequence in foods (Klein, 2002;Levin, 2004). The simple chemistry of a realtime assay is based on the use of two Xuorescent dyes: a reporter (R) and a quencher (Q), attached to the 5Ј and 3Ј ends, respectively, of a Taqman speciWc probe (Applied Biosystem).…”