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SummaryA microcalorimetric method has been developed for the identification of 9 representative strains of lactic acid bacteria used in the dairy industry. A chemically defined medium containing several carbohydrates gave reproducible and characteristic growth patterns. The technique is proposed as a rapid alternative method for the identification of bacteria selected for commercial use.

An image analysis system which incorporates a microscope, video camera, monitor, and Apple computer and which uses image area to count Saccharomyces cerevisiae cells is described and evaluated. Yeast cell suspensions of densities of up to 100 x 106 cells per ml can be counted when viewed in the counting chamber of a hemacytometer. The yeast image area measured depends upon the light intensity used to illuminate the yeast cells, the sharpness of the image focused on the monitor, and the grey level selected when scanning the digitized image on the mQnitor of the Apple computer, all of which can be controlled. The image area also depends upon the yeast strain and medium in which the culture is grown, but it is not affected by the concentration of sugar or ethanol in which the yeast cells are suspended. Yeast growth measured by image analysis can be calibrated to give results similar to those obtained with hemacytometer counting. Yeast cells can be counted in the presence of high cell densities of bacteria by adjusting the grey level at which the digitized image is scanned.

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