Calorimetric identification of several strains of lactic acid bacteria

Abstract

SummaryA microcalorimetric method has been developed for the identification of 9 representative strains of lactic acid bacteria used in the dairy industry. A chemically defined medium containing several carbohydrates gave reproducible and characteristic growth patterns. The technique is proposed as a rapid alternative method for the identification of bacteria selected for commercial use.

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

“…However more information can be obtained. Firstly monitoring growth rate and production of heat might allow comparing or typing different starter cultures [128], especially for milk products. On the other hand, if the same starter culture is used as a ''bioindicator'', one can easily compare the properties of the milk tested [122,123,129].…”

Application of differential scanning calorimetry in food research and food quality assurance

“…11) illustrates from our laboratory changes of heat flow recorded during isothermic incubation of Streptococcus bovis and Bacillus cereus cultures, respectively. Microcalorimetry of microbial growth in various media provides informative data regarding microbial metabolism and may assist identification or differentiation of taxonomic entities [98][99][100]. Thermograms obtained by modern thermal bioactivity monitors allows calculation of growth and activation energy parameters [101,102].…”

Flow Microcalorimetric Studies of Yeast Growth: Fundamental Aspects

“…The argument for the use of a complex medium centred around the presence of multiple peaks and troughs in the derived p-t curves. The use of combinations of sugars in a defined medium can, however, yield the same effects (Fujita et al 1978). Indeed the taxonomic classification of yeast species is frequently based upon the observation of differing rates of fermentation and/or the ability to ferment certain sugars.…”

“…In a limited study (three experiments using inocula of uncontrolled viability, single organism, complex medium) Johansson et al (1975) used computer evaluation of microcalorimetric data in an attempt to eliminate the effects of inoculum density. Schaarschmidt & Lamprecht (1976), Fujita et al (1978 and Monk (1978) have implied that for a given o r g a n i s m p t curves are sensitive to many experimental parameters, such as inoculum properties, 0021-8847/79/060527+ 11%01.00/0…”