Fate of Carbon Passing Through the Glucose Pool of Rumen Digesta

Walker, D. J.; Monk, Paul R.

doi:10.1128/aem.22.5.741-747.1971

Cited by 10 publications

(3 citation statements)

References 12 publications

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“…It is also possible that glucose was metabolized by microbial populations separate from those that were responsible for the flow of carbon from cellulose in the bioreactor. For example, Walker and Monk [14] found that [U-14C]glucose added carrier-free to ovine rumen fluid was converted primarily to acetate and CO 2 similar to the results of this study, even though significant amounts of propionate and butyrate were presumably being produced from plant carbohydrate. King and Klug [15] found significant amounts of lactate, propionate, and butyrate were produced by Wintergreen Lake sediments from [14C]glucose, indicating that coupling was not as tight in this system.…”

Section: Discussionsupporting

confidence: 83%

“…Glucose turnover in the bioreactor was a very dynamic process with turnover occurring within a few seconds. Walker and Monk [14] found a similarly short turnover time for [14C]glucose incubated with rumen fluid. King and Klug [15], in their studies on glucose turnover in anaerobic sediments of the eutrophic Wintergreen Lake, estimated turnover times for [14C]glucose added to sediments to be near 1 min, with 90% of the label taken up within 5 min.…”

Section: Discussionmentioning

confidence: 87%

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Patterns of carbon flow from glucose to methane in a thermophilic anaerobic bioreactor

Zinder

1986

FEMS Microbiology Letters

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[U‐14C]Glucose, added carrier‐free to sludge from a thermophilic anaerobic bioreactor being fed a lignocellulose waste, was rapidly turned over with less than one‐third of the original radiolabel remaining as glucose after 5 s of incubation. The primary labeled products found were [14C]acetate and 14CO2, which were in a ratio near 2:1. Further incubation resulted in the disappearance of [14C]acetate and the appearance of an equivalent amount of label as 14CH4 and 14CO2. No significant production of [14C]propionate, butyrate, lactate, or ethanol was detected from [14C]glucose, even if these potential intermediates (unlabeled) were added to the sludge at a concentration of 1 mM to trap any label entering their pools. Addition of 0.8 atm (80 kPa) of H2 to the headspace over sludge resulted in some accumulation of [14C]lactate and a corresponding decrease in [14C]acetate produced from [14C]glucose. Production of [14C]propionate, butyrate and ethanol were still not significant in the presence of H2. Incubation of sludge for 1 h in the presence of hydrogen resulted in increases in the lactate and formate concentrations, but not those of propionate, butyrate, or ethanol. These results demonstrate that glucose was metabolized directly to acetate, CO2, and H2 by the microbial populations in the bioreactor with little carbon from glucose flowing through other intermediates, indicating a high degree of coupling between glucose fermentation and hydrogen uptake. The short‐term response of these microbial populations to elevated H2 partial pressures was to increase lactate production.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 87%

Patterns of carbon flow from glucose to methane in a thermophilic anaerobic bioreactor

Zinder

1986

FEMS Microbiology Letters

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“…An increase in glucose dose by varying the infusion time (Table 2) or the amount of glucose added (Table 3) resulted in a decrease in Hf. It is possible that in the presence of large amounts of glucose, maintenance requirements of the rumen microbes are met and a shift from catabolic to anabolic processes is likely, resulting in intracellular accumulation of substrates or synthesis of storage polysacarides (Walker & Monk, 1971).…”

Section: Discussionmentioning

confidence: 99%

Effect of glucose on fermentation heat in sheep rumen fluid in vitro

Arieli

1986

Br J Nutr

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1. Heat production rate (a of rumen fluid was measured in a direct calorimeter, Basal a of samples of 15 ml 2. Addition of glucose (04-6.4 mg/sample) was followed by a dose-dependent increase in H . Maximal H was 3. Expression of fermentation heat (Hf ; kJ/mol substrate added) against glucose dose indicated an asymptotic 4. Maximal H f (at infinite dilution) agreed with stoichiometric calculations whereas minimal Hf suggested a rumen fluid mixed with 45 ml buffer was 0.4 mW/ml rumen fluid.

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Heterotrophic Utilization and Regeneration of Nitrogen

Billen¹

1984

Heterotrophic Activity in the Sea

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Fate of Carbon Passing Through the Glucose Pool of Rumen Digesta

Cited by 10 publications

References 12 publications

Patterns of carbon flow from glucose to methane in a thermophilic anaerobic bioreactor

Patterns of carbon flow from glucose to methane in a thermophilic anaerobic bioreactor

Effect of glucose on fermentation heat in sheep rumen fluid in vitro

Heterotrophic Utilization and Regeneration of Nitrogen

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