Oligonucleotide fingerprinting of genomic DNA from oral isolates of four different Candida species other than C. albicans and atypical chlamydospore-positive isolates from human immunodeficiency virus (HIV)-positive individuals and AIDS patients was investigated as a means for differentiating between isolates within individual species. Oligonucleotides composed of simple repetitive sequence motifs, including (GACA)4, (GATA)4, (GGAT)4, (GTG)5, and (GT)8, all yielded fingerprints suitable for strain segregation of 8 C. tropicalis isolates, 12 Torulopsis (Candida) glabrata isolates, 8 atypical Candida isolates, and, except for (GATA)4, 2 C. krusei isolates. This technique makes it possible to carry out sequential hybridization of filter-bound DNA with each probe in turn and so generate several distinct DNA fingerprints of the same DNA sample. However, none of the probes yielded fingerprints suitable for strain segregation with three C. parapsilosis isolates. The (GATA)4 probe was also used to detect restriction fragment length polymorphisms among a genetically closely related group of atypical Candida isolates on primary isolation from an additional HIV-infected patient. These chlamydospore-positive atypical Candida isolates were sucrose positive, were of C. albicans serotype A, hybridized weakly with the C. albicans-specific mid-repeat sequence probe 27A, and yielded fingerprint profiles by random polymorphic DNA analysis that were distinct from those derived from C. albicans isolates. The C. stellatoidea ex-type strain NCPF 3108 was indistinguishable from the atypical Candida isolates in all these tests and also yielded an identical carbohydrate and nitrogen source assimilation profile by using the ID 32C yeast identification system.
Now available in this country, the outstanding SP.500 uv and visible range spectrophotometer. Try it. The first thing you'll notice is that the controls are mounted on a sloping panel -only Unicam can be operated accurately and comfortably by a seated operator. Saves fatigue, permits continuous operation.Turn on the instrument. Notice how the dials and other controls move precisely, smoothly through their cycles, giving the operator a sense of confidence. The heart of a high-quality spectrophotometer is an efficient monochromator. Check Unicam's! See how it combines excellent transmission characteristics, and narrow spectral bandwidth with a minimum of stray light. See how the universal cell housing focuses the light beam precisely for microcell workanother exclusive feature. But don't stop with the operation. Lift the cover. See the neatly soldered controls, the precision workmanship throughout. Note the convenient dual source housingstandard equipment on Unicam. The prism is of fused silica, another major improvement to extend the working range to 186 mu.We'll place a Unicam SP.500 in your laboratory on trial so you can prove its many advantages on both routine and special analytical work. Before you spend money on a spectrophotometer, see how much more you get with a Unicam, at lower cost. Write, wire or phone for a Unicam SP.500 trial without cost or obligation. Visit Booths 16 and 73 at the Pittsburgh ConferenceNow available in this country, the outstanding SP.500 uv and visible range spectrophotometer. Try it. The first thing you'll notice is that the controls are mounted on a sloping panel -only Unicam can be operated accurately and comfortably by a seated operator. Saves fatigue, permits continuous operation.Turn on the instrument. Notice how the dials and other controls move precisely, smoothly through their cycles, giving the operator a sense of confidence. The heart of a high-quality spectrophotometer is an efficient monochromator. Check Unicam's! See how it combines excellent transmission characteristics, and narrow spectral bandwidth with a minimum of stray light. See how the universal cell housing focuses the light beam precisely for microcell workanother exclusive feature. But don't stop with the operation. Lift the cover. See the neatly soldered controls, the precision workmanship throughout. Note the convenient dual source housingstandard equipment onUnicam. The prism is of fused silica, another major improvement to extend the working range to 186 mu.We'll place a Unicam SP.500 in your laboratory on trial so you can prove its many advantages on both routine and special analytical work. Before you spend money on a spectrophotometer, see how much more you get with a Unicam, at lower cost. Write, wire or phone for a Unicam SP.500 trial without cost or obligation. Visit Booths 16 and 73 at the Pittsburgh ConferenceNow available in this country, the outstanding SP.500 uv and visible range spectrophotometer. Try it. The first thing you'll notice is that the controls are mounted on a sloping panel -only...
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