The hsp70 family of major stress proteins is composed of several different members exhibiting similar structural and functional properties. In order to obtain an antiserum with wide epitope reactivity, rabbits were immunized with a mixture of native and denatured hsp70 purified from bovine muscle by ATP-affinity chromatography. Screening for antibody specificity was performed by a "sandwich" enzyme linked immunosorbent assay (ELISA). Immunoprecipitation and immunoblotting analyses demonstrated that the polyclonal antiserum obtained by us and a monoclonal antibody raised against a different preparation of antigen recognized the same determinant on the native hsp70 molecule (inducible form). With a different specificity the polyclonal antiserum recognized only the denatured monomers of the other members of the hsp70 family. These results are discussed in relation to the immunological features of the hsp70 molecule and to the development of an immunoassay for the detection of hsp70 in cell and tissue extracts.
For many decades, the problem of chronic tonsillitis still does not lose its relevance. However, some aspects of the etiology and pathogenesis of chronic tonsillitis remain unexplored. This article presents the results of a study of the bacterial flora and structural components of the tissues of the palatine tonsils in chronic decompensated tonsillitis and appendix in patients with acute tonsillitis, as well as a comparison of some indicators of the general clinical analysis of peripheral blood in patients with these types of pathology. Chronic tonsillitis occurred in the history of patients with acute appendicitis in 78% of cases, which is ten times the frequency of its detection in the urban population. In all patients with acute appendicitis, palatine tonsil hypertrophy was detected, mainly of the III degree, as well as the presence of liquid or thick caseous pus in the gaps. In chronic decompensated tonsillitis, an increase in the seeding of the palatine tonsils of Streptococcus pyogenes occurs, and in acute appendicitis, Staphylococcus aureus and Streptococcus pyogenes appear on the background of Escherichia coli. Unidirectional changes in the areas of structural components of palatine tonsils in patients with chronic decompensated tonsillitis and appendix in patients with acute appendicitis were revealed. Based on the data obtained, we can assume the similarities and interconnections of the pathogenesis of chronic decompensated tonsillitis and acute appendicitis.
Introduction. Based on the fact that all classifications are a method of generalization, most authors in their own research, as a rule, use several classifications. On the one hand, this allows obtaining the most accurate characterization of the disease and substantiating the methods of its treatment. On the other hand, it significantly reduces the possibility of comparing various data and an adequate assessment of the etiological and pathogenetic mechanisms of the disease. The results of a long-term study of laryngeal diseases, considering one of the main etiological and pathogenetic causes, which is a violation of the adaptive-trophic function of the autonomic nervous system (ANS), allow, to some extent, eliminating the existing difficulties. Objective. Transformation of the classification systematics of dysphonia considering the neurogenic link of pathogenesis. Materials and methods. A comprehensive clinical and physiological examination of 125 patients aged 18 to 75 years was carried out for voice disorders with clinical diagnoses of “polyp of the vocal fold” (60), “chronic laryngitis” (20), “hypotonic type of functional dysphonia in combination with laryngeal hypersensitivity” (45). During the examination, the medical history was studied, an objective examination of the upper respiratory tract according to generally accepted methods and with the help of videostroboscopic unit, cytological examination of impression smears from the affected laryngeal area, and a study of the functional state of the ANS as well as the surgical material, including histological and histochemical examination of the removed tissue, were carried out. Surgical intervention to remove pathological formations was performed in 60 cases in patients with polyps of the vocal folds and in 3 cases in patients with chronic laryngitis. Results. The conducted studies have shown that the changes that lead to chronic hypertrophic laryngitis, functional dysphonia, tumor-like hyperplastic-dystrophic processes as well as the changes in the sensitivity of the larynx by the type of «denervation hypersensitivity» are largely associated with neurogenic, including neurodystrophic, pathogenicity factors. In groups of patients with inadequate autonomic maintenance of activity, where it is a question of violations of the functional activity of the ANS (insufficient or excessive autonomic maintenance of activity), pathological dystrophic changes of varying severity develop in the mucous membrane of the larynx. Conclusion. The proposed classification of dysphonia is based on the clinical and morphological features of laryngeal diseases, including the neurodystrophic component of the development of the pathological process.
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