In conclusion, the nested RT-PCR technique identified the presence of transcript encoding ERalpha mRNA in human skeletal muscles. Semi-quantification did not reveal gender difference.
The purpose of this study was to investigate the effects of active recovery (AR) on plasma lactate concentration [La] and anaerobic power output as measured during repeated bouts of intense exercise (6 s) against increasing braking forces. Ten male subjects performed two randomly assigned exercise trials: one with a 5-min passive recovery (PR) after each exercise bout and one with a 5-min active recovery (AR) at a workload corresponding to 32% of maximal aerobic power. Blood samples were taken at rest, at the end of each exercise bout (S1) and at the 5th minute between bout-recovery (S2) for plasma lactate assay. During the tests, [La]S1 was not significantly different after AR and PR, but [La]S2 was significantly lower after AR for power outputs obtained at braking forces 6 kg (5.66 +/- 0.38 vs 7.56 +/- 0.51 mmol.l-1) and peak anaerobic power (PAnP) (6.73 +/- 0.61 vs 8.54 +/- 0.89 mmol.l-1). Power outputs obtained at 2 and 4 kg did not differ after AR and PR. However, when compared with PR, AR induced a significant increase in both power outputs at 6 kg (842 +/- 35 vs 798 +/- 33 W) and PAnP (945 +/- 56 vs 883 +/- 58 W). These results showed that AR between bouts of intensive exercise decreased blood lactate concentration at high braking forces. This decrease was accompanied by higher anaerobic power outputs at these forces.
We investigated the aerobic and anaerobic contributions to performance during the Wingate test in sprint and middle-distance runners and whether they were related to the peak aerobic and anaerobic performances determined by two commonly used tests: the force-velocity test and an incremental aerobic exercise test. A group of 14 male competitive runners participated: 7 sprinters, aged 20.7 (SEM 1.3) years, competing in 50, 100 and 200-m events and 7 middle-distance runners, aged 20.0 (SEM 1.0) years, competing in 800, 1,000 and 1,500 m-events. The oxygen uptake (VO2) was recorded breath-by-breath during the test (30 s) and during the first 20 s of recovery. Blood samples for venous plasma lactate concentrations were drawn at rest before the start of the test and during the 20-min recovery period. During the Wingate test mean power (W) was determined and three values of mechanical efficiency, one individual and two arbitrary, 16% and 25%, were used to calculate the contributions of work by aerobic (Waer,ind,16%,25%) and anaerobic (Wan,ind,16%,25%) processes. Peak anaerobic power (Wan,peak) was estimated by the force-velocity test and maximal aerobic energy expenditure (Waer,peak) was determined during an incremental aerobic exercise test. During the Wingate test, the middle-distance runners had a significantly greater VO2 than the sprinters (P < 0.001), who had significantly greater venous plasma lactate concentrations (P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
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