The genetics of esterase-28, the major esterase of cauda epididymidis of the house mouse, has been studied after separation by polyacrylamide gel electrophoresis and isoelectric focusing. Four phenotypes are distinguished. Segregation of Es-28 in two backcross series indicated linkage to Es-1, Es-9, and Es-22. The Es-28 locus was placed into esterase cluster 1 on chromosome 8.
Genetic variation of a carboxylesterase isozyme (EC 3.1.1.1) of the house mouse, designated ES-23, is described. ES-23 was found in kidney, liver, and intestine. The isozyme was resistant to inhibition by 10(-3) mol/liter eserine and was stained using alpha-naphthyl butyrate or 5-bromoindoxyl acetate as substrate. Five different phenotypes, ES-23A to ES-23E, could be distinguished by disc electrophoresis and by isoelectric focusing. ES-23 is controlled by a structural locus situated within the esterase gene cluster 2 on chromosome 8. An analysis of allele distribution among different strains suggested a separate structural locus for the isozyme, Es-23e, which is closely linked to the loci Es-2, Es-5, Es-7, and Es-11. Of the five phenotypes, only ES-23B was expressed in lung. This variation is apparently controlled by a cis-acting regulatory element, presumably a temporal locus, Es-23t, closely linked to the presumed structural locus Es-23e.
Genetic variation of a codominantly inherited pancreas protease, designatedFrom two backcross series, including a total of 274 animals, the gene order (Es-1, Es-9)--3.9 -+ 1.7%--Got-2--3.9 +-1.7%--(Es-2, Es-7, Es-23)--0. 7 -+ 0.5%--Ctra-l--6.3 __ 2.2%--Prt-2 was established.
A six-point cross was carried out to determine the gene order and distances among loci on mouse chromosome 9. Our results are consistent with the following arrangement: centromere - Lap-1 - (1.2 ± 0.8) - Es-17 - (3.0 ± 1.0) - Ups - (1.3 ± 0.7) - Alp-1 - (23.1 ± 3.4) - Mod-1 - (10.9 ± 2.6) - Acy-1. This study provides the first estimate of the distances between Es-17, Ups and Alp-1. Exceptions to the preferred association of alleles of Es-17 and Ups have been found in three feral populations and one inbred strain. Evidence is presented for the homology of this chromosome region with the ESA4 - UPS - APO-AI region on the long arm of human chromosome 11.
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