Esterase-23 (ES-23): Characterization of a new carboxylesterase isozyme (EC 3.1.1.1) of the house mouse, genetically linked to ES-2 on chromosome 8

Deimling, Otto H. von

doi:10.1007/bf00499472

Cited by 19 publications

(2 citation statements)

References 16 publications

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“…It is distinct from the e3 locus (Hughes and Raftos, 1985). Since clusters of esterase loci occur in a number of species, including the rat (Hedrich and von Diemling, 1987), the rabbit (van Zutphen et aL, 1987), and probably the mouse (Peters, 1982;von Diemling, 1984), it seems possible that in Lucilia these chromosome 4 loci form part of an esterase cluster. To determine this will require mapping experiments involving all three loci.…”

Section: Discussionmentioning

confidence: 97%

Genetics of the hemolymph esterases ofLucilia cuprina (Diptera: Calliphoridae)

Lai-Fook

Smith

1992

Biochem Genet

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When hemolymph from adults of Lucilia cuprina was partitioned on native polyacrylamide gels, nonspecific esterase staining demonstrated 10 bands with up to six bands in an individual. The bands derive from alleles at two loci, EHA (five alleles) and EHB (four alleles). EHA is located on chromosome 4, 16.3 map units from sv (singed vibrissae) and 22.1 map units from ra (radial vein gaps). EHB is located on chromosome 5, 34.0 map units from to2 (topaz2 eyes) and 7.2 map units from mv (M1-veinless).

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Section: Discussionmentioning

confidence: 97%

Genetics of the hemolymph esterases ofLucilia cuprina (Diptera: Calliphoridae)

Lai-Fook

Smith

1992

Biochem Genet

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“…Large numbers of genetically wellcharacterized inbred strains and recombinant inbred strains exist, the linkage map is well studied, and several closely related wild species of mouse are available for study [Felder, 19801. Tissue-specific differences in the relative intensities of allelic isozyme bands in heterozygous mice have been reported. 1) In two species of wild house mouse, Mus cusruneus and Mus molossinus, von Deimling [1984] found an allele of carboxylesterase (ES-23B) that was expressed in lung while an alternate allele for the same locus was not expressed in lung. At this locus, no alleles found in laboratory mice showed such a tissue-specific mode of expression.…”

Section: Introductionmentioning

confidence: 99%

Murine “housekeeping” enzyme (genetic locus:Idh‐1) is regulated in an allele‐specific manner

Zitnik

Martin

1987

Dev. Genet.

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The murine "housekeeping" enzyme, cytosolic NADP-isocitrate dehydrogenase (E.C.1.1.1.42) (genetic locus: Idh-1), exhibited a complex pattern of allele-specific expression. Protein electrophoresis on cellulose-acetate gels and determination of relative enzymatic activity by means of densitometry revealed that in heart tissue (but not liver tissue) of certain hybrid crosses the AA-homodimer was underrepresented relative to total enzymatic activity, and the degree of underrepresentation changed during development. In mixtures of homozygous tissue extracts of heart tissue (but not liver tissue) the AA-homodimer was underrepresented relative to the BB-homodimer. Relative activity of allelic isozymes varied as a function of tissue (heart versus liver), age, and the parental source of the Idh-1 alpha allele, but did not vary as a function of sex. Allele-specific expression was also exhibited in kidney tissue of the same animals. In adult male kidney tissue extracts from heterozygotes, the AA-homodimer was underrepresented relative to total enzymatic activity; in adult female kidney tissue extracts from heterozygotes, a more codominant phenotype was observed. Tissue extracts from immature hybrid animals exhibited a phenotype midway between the adult male and adult female phenotypes. Tissue extracts from castrated males exhibited a phenotype equivalent to that seen in females. Relative activity of allelic isozymes in kidney varied as a function of age and sex, but did not vary as a function of the parental source of the Idh-1 alpha allele. While cytosolic NADP-IDH is a "housekeeping" enzyme, expressed in multiple tissues of the mouse, differences in the relative intensities of allelic isozyme bands provide evidence for tissue- and stage-specific regulatory variation.

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Micro two‐dimensional gel electrophoresis of serum and testis esterases from different strains of mice

Abou-Haïla

1990

Electrophoresis

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Two-dimensional electrophoresis with time-dependent polyacrylamide gradient gel electrophoresis (PAGGE) in the second dimension was applied to the separation of native molecular forms of esterases from serum and testis of four strains of mice (C57BL/6J, Swiss OF1, F1 hybrid derived from these two populations and Tfm). In Phast System, a modified pH 3-9 gradient, a linear 8-25% gel gradient and a migration time corresponding to 300 Vh, were found to provide the best conditions for esterase analysis. About 70 esterase-active fractions could be separated with good reproducibility. The variants were characterized by their pI (3.9-7.35), their relative mobility and the visual estimation of their susceptibility towards neuraminidase and different esterase inhibitors. In the two tissues, the distribution of the esterase variants corresponded to a 50-500 kDa molecular mass range of calibration proteins, but most of the serum and testis-specific isoforms were confined to the 59-72 kDa range. All serum variants contained a terminal N-acetylneuraminic acid residue, whereas only the testicular esterases in common with those in serum appeared sensitive to neuraminidase. Cholinesterases with a low relative mobility and carboxylesterases with a high relative mobility were detected in serum, while carboxylesterases accounted for the greatest part in the testis which also contained cholinesterases and acetylesterases. Minor interspecies differences were found between C57BL/6J and Swiss OF1 esterases. The expression of two variants which differed between these two species seemed intermediate for the hybrid originating from these two populations. Two new spots were detected in the two-dimensional map of esterases from the strain bearing the Tfm mutation.

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Esterase-23 (ES-23): Characterization of a new carboxylesterase isozyme (EC 3.1.1.1) of the house mouse, genetically linked to ES-2 on chromosome 8

Cited by 19 publications

References 16 publications

Genetics of the hemolymph esterases ofLucilia cuprina (Diptera: Calliphoridae)

Genetics of the hemolymph esterases ofLucilia cuprina (Diptera: Calliphoridae)

Murine “housekeeping” enzyme (genetic locus:Idh‐1) is regulated in an allele‐specific manner

Micro two‐dimensional gel electrophoresis of serum and testis esterases from different strains of mice

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