Cellulose has enormous potential as a renewable energy source and the application of cellu lases in the conversion of cellulo lytic bio mass may provide great economical benefits; in addition, cellulases can be used in a wide range of industrial applications. Given the b iotechnological importance of cellulases, the aim of this study was to evaluate the production of cellulases by endophytic fungi o f the genus Fusarium oxysporum isolated fro m Baccharis dracunculifolia D.C. (Asteraceae). The studies were conducted using a basic substrate of sugarcane bagasse that was pretreated for complete removal of the sugar content. The material was dried at 28°C for 96 days and quantified every seven days after 25 days of fermentation. To quantify the en zy mes, the indirect spectrophotometric method was used, with DNS reagent. The results showed that the greatest peak of enzyme production was at 55 days of fermentation, with a yield of 55.21 ± 10.54 IU/g of fermented substrate, at pH 5.96. Thus, it can be concluded that the fungus Fusarium oxysporum is a producer of cellu lase enzy mes.
Cellu lases are enzy mes of great industrial interest, which are used in the food, pharmaceuticals, cosmetics, detergents and textile industries. Applications include the bleaching of pulp in the paper industry, the production of dissolved pulp, waste water treat ment and recycling of waste paper. Studies have been carried out regarding the ability of microorganis ms to produce enzymes, using available and affordable substrates. The aim of this study was to evaluate the ability of the endophytic fungus Aspergillus flavus, strain (D2-FB) to produce cellu lase. The studies were carried out using a substrate of sugarcane bagasse supplemented with 1% cellobiose and carboxy methylcellulose. The material was kept in an oven at 27º C fo r 69 days and the enzymes were measured every 7 days. To quantify the enzy mes, the DNS method was adopted. The results showed that the highest production occurred at 32 days, with a production of 33.15 ± 7.96 U/g of substrate. After this period, the en zy me production decreased gradually up to 3.06 ± 0.53 U/g. Based on these results, it can be concluded that the endophytic fungus A. flavus, strain (D2-FB), is a producer of cellu lases.
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