Production of Cellulases by the Endophytic Fungus Fusarium oxysporum

Becker, Onofre Sideney

doi:10.5923/j.microbiology.20130304.01

Cited by 13 publications

(5 citation statements)

References 9 publications

(9 reference statements)

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“…Hasil pengujian didapatkan ketiga sampel positif memiliki zona bening (halo) yang terlihat disekitar koloni (Gambar 4). Dalam penelitian lainnya disebutkan bahwa Fusarium graminearum (Davilla et al, 2022, Kikot et al, 2010 dan Fusarium oxysporum (Onofre, 2013), diketahui dapat menghasilkan enzim selulase, sedangkan Colletotrichum coccodes belum pernah dilaporkan menghasilkan enzim selulase.…”

Section: Seleksi Fungi Endofit Penghasil Selulaseunclassified

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Dhia Salsabila Hakim,

Kasiamdari

2023

bib

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Abstrak: Fungi endofit menjadi perhatian di industri bioetanol karena peranannya dalam sekresi metabolit sekunder berupa enzim selulolitik yang diketahui lebih besar daripada bakteri. Sekresi enzim metabolik pada fungi endofit tergantung pada inang yang ditempatinya. Tanaman herbal dikenal sebagai tempat hidup berbagai fungi endofit. Daun sirih hijau (Piper betle L.) merupakan salah satu tanaman herbal yang dikenal memiliki kandungan metabolit tinggi. Penelitian ini bertujuan untuk mengidentifikasi fungi endofit yang hidup pada P. betle, melakukan uji selulolitik fungi hasil identifikasi serta mengetahui fungi endofit yang memiliki aktivitas selulolitik tebesar. Uji selulolitik dilakukan dengan dua tahap yaitu seleksi dengan Congo Red serta aktivitas selulolitik dihitung dengan kadar gula reduksi. Hasil penelitian didapatkan tiga spesies fungi dari P. betle yaitu Fusarium graminearum, Fusarium oxysporum dan Colletotrichum coccodes. Ketiga sampel yang diujikan menunjukkan positif menghasilkan enzim selulase yang ditandai dengan adanya zona bening di sekitar koloni. Fungi endofit penghasil enzim selulase terbesar adalah spesies F. oxysporum dengan angka 0,033 U/ml.

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Section: Seleksi Fungi Endofit Penghasil Selulaseunclassified

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Dhia Salsabila Hakim,

Kasiamdari

2023

bib

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“…Enzymatic hydrolysis of cellulosic biomass offers an attractive alternative for the generation of sugars, which can serve as the raw materials in various economically relevant processes, such as cotton processing, paper recycling, juice extraction, enzymatic detergents, and animal food additives. The genera Aspergillus, Trichoderma, Humicola, Penicillium, Fusarium, and Phanerochaete are widely used in industrial enzyme production [23]. However, the high production cost and the low yield of cellulase are still the major constraints in the economics of the process, and the discovery of novel fungal species secreting cellulases is still an emerging area of research to develop economically competitive bioprocess strategies applicable on a large scale [46].…”

Section: Yucatanensis (Cml4014mentioning

confidence: 99%

“…Endophytic fungi occupy a relatively unexplored area in microorganism isolation, and thus represent a new source for obtaining enzymes with different potentials. Studies have shown that endophytic fungi can produce amylases, lipases, proteases, pectinases, and cellulases [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Enzyme Production by Induratia spp. Isolated from Coffee Plants in Brazil

Monteiro

Tavares

Nery

et al. 2020

Braz. arch. biol. technol.

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Endophytic fungi belonging to the genus Muscodor now transferred to Induratia are known producers of bioactive volatile organic compounds (VOCs) with many industrial applications. However, the members of this genus have rarely been reported to produce non-volatile metabolites including enzyme. Enzymes of the endophytes are degraders of the polysaccharides available in the host plants and the knowledge of enzyme production by Induratia spp. may provide insights into their possible biotechnological applications. The aim of this study was to evaluate the activity of amylase, cellulase, lipase, pectinase, phytase, protease, endo β-1,4 glucanase and exo β-1,4 glucanase enzymes produced by fungi of the species Induratia coffeana, Induratia yucatanensis and Induratia sp. isolated from organic coffee plants. All Induratia spp. were able to produce the extracellular enzymes cellulase, pectinase, protease, and phytase. Eight fungi were able to produce lipase and four produced amylase. The specific activity of endo β-1, 4 glucanase and exo β-1,4 glucanase enzymes were detected for 9 and 8 endophytic fungi, respectively. This work demonstrated for the first time, the array of enzymes produced by Induratia spp. isolated from Coffea arabica in organic systems in Brazil.

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“…Keeping in view the wide use of cellulase in industry, there is need for better and efficient cellulase [1,2].…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression and characterization of mutant cellulase from indigenous strain of Aspergillus niger

Ahmad,

Zafar,

Anwar

et al. 2024

Preprint

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The current study highlights the effect of mutagenesis on endoglucanase B activity of indigenous strain of Aspergillus niger and its heterologous expression studies in the pET28a+ vector. The physical and chemical mutagens were employed to incorporate mutations in A. niger. For determination of mutations, mRNA was isolated followed by cDNA synthesis and cellulase gene was amplified, purified and sequenced both from native and mutant A. niger. On comparison of gene sequences, it was observed that 5 nucleotide base pairs have been replaced in the mutant cellulase. The mutant recombinant enzyme showed 4.5 times higher activity (428.5 µmol/mL/min) as compared to activity of native enzyme (94 µmol/mL/min). The mutant gene was further investigated using Phyre2 and I-Tesser tools which exhibited 71% structural homology with Endoglucanase B of Thermoascus aurantiacus. The root mean square deviation (RMSD), root mean square fluctuation (RMSF), solvent accessible surface area (SASA), radius of gyration (Rg) and hydrogen bonds analysis were carried at 35 °C and 50 °C to explore the integrity of structure of recombinant mutant endoglucanase B which corresponded to its optimal temperature. Hydrogen bonds analysis showed more stability of recombinant mutant endoglucanase B as compared to native enzyme. Both native and mutant endoglucanase B genes were expressed in pET 28a+ and purified with nickel affinity chromatography. Theoretical masses determined through ExPaSy Protparam were found 38.7 and 38.5 kDa for native and mutant enzymes, respectively. The optimal pH and temperature values for the mutant were 5.0 and 50 °C while for native these were found 4.0 and 35 °C, respectively. On reacting with carboxy methyl cellulose (CMC) as substrate, the mutant enzyme exhibited less Km (0.452 mg/mL) and more Vmax (50.25 µmol/ml/min) as compared to native having 0.534 mg/mL as Km and 38.76 µmol/ml/min as Vmax. Among metal ions, Mg2+ showed maximum inducing effect (200 %) on cellulase activity at 50 mM concentration followed by Ca2+ (140%) at 100 mM concentration. Hence, expression of a recombinant mutant cellulase from A. niger significantly enhanced its cellulytic potential which could be employed for further industrial applications at pilot scale.

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Production of Cellulases by the Endophytic Fungus Fusarium oxysporum

Cited by 13 publications

References 9 publications

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Enzyme Production by Induratia spp. Isolated from Coffee Plants in Brazil

Heterologous expression and characterization of mutant cellulase from indigenous strain of Aspergillus niger

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