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The mean width (n = 5) of the cephalic capsule instar of Alphitobius diaperinus was determined. The larvae were reared at 27ºC (+ 0.1ºC). The result showed that A. diaperinus has eight larval instars. The head capsule of the 1 st instar larvae measured x = 0.228 (SD = 0.0192) and the last instar larval measured x = 1.339 (SD = 0.0436). The developmental rate, determined by Dyar-Hutchinson's rule, was 1.29. These data may be useful for studies on phenology and age structure of A. diaperinus in the field.Key words: lesser mealworm, larval instars, cephalic capsule, caged layer manure. RESUMO Caracterização dos estádios larvais de Alphitobius diaperinus (Panzer) (Coleoptera:Tenebrionidae) usando a largura da cápsula cefálica O valor médio da largura da cápsula cefálica de Alphitobius diaperinus foi verificado de acordo com seus estádios larvais, sendo medidos cinco indivíduos para cada estádio. O experimento foi conduzido em temperatura constante de 27ºC (+ 0,1ºC). Os resultados obtidos mostraram que as larvas atingem oito estádios, com média de 0,228 mm (SD = 0,0192) para a larva recém-eclodida, até 1,339 mm (SD = 0,0436) no 8 o estádio. Também foi observado que o fator de crescimento das larvas corresponde à regra de Dyar-Hutchinson, apresentando média de 1,29 para razões de crescimento ao longo do desenvolvimento. Esses dados poderão ser utilizados em estudos de fenologia e estruturação etária de populações de A. diaperinus no campo.Palavras-chave: cascudinho, ínstares larvais, cápsula cefálica, esterco de aves poedeiras.

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The European mink (Mustela lutreola) is a riparian mustelid, considered one of the most endangered carnivores in the world. Alpha, beta and gammaherpesviruses described in mustelids have been occasionally associated with different pathological processes. However, there is no information about the herpesviruses species infecting European minks. In this study, 141 samples of swabs (oral, conjunctival, anal), faeces and tissues from 23 animals were analysed for herpesvirus (HV) using a pan-HV-PCR assay. Two different, potentially novel, gammaherpesvirus species were identified in 12 samples from four animals (17.3%), and tentatively named Mustelid gammaherpesvirus-2 (MUGHV-2) and MuGHV-3. Gross examination was performed on dead minks (n = 11), while histopathology was performed using available samples from HV-positive individuals (n = 2), identifying several neoplasms, including B-cell lymphoma (identified by immunohistochemistry) with intralesional syncytia and intranuclear inclusion bodies characteristic of HV (n = 1), pulmonary adenocarcinoma (n = 1), and biliary (n = 1) and preputial (n = 1) cystadenomas, as well as other lesions (e.g., axonal vacuolar degeneration [n = 2] and neuritis [n = 1]). Viral particles, consistent with HVs, were observed by electron microscopy in the mink with neural lymphoma and inclusion bodies. This is the first description of neoplasms and concurrent gammaherpesvirus infection in European minks. The pathological, ultrastructural and PCR findings (MuGHV-2) in the European mink with lymphoma strongly suggest a potential role for this novel gammaherpesvirus in its pathogenesis, as it has been reported in other HV-infected species with lymphoma. The occurrence of neural lymphoma with intralesional syncytia and herpesviral inclusions is, however, unique among mammals. Further research is warranted to elucidate the potential oncogenic properties of gammaherpesviruses in European mink and their epidemiology in the wild population.

Avian scavengers that typically include game birds and mammals in their diets are at risk of lead poisoning from ingestion of carcasses with fragmented or residual lead ammunition that is used in hunting. Thus, lead may be one of the threats that the griffon vulture ( Gyps fulvus ) faces in the Iberian Peninsula and particularly in Portugal, where their conservation status is considered to be near-threatened. This is the first report that details 3 cases of lead poisoning, associated with the ingestion of lead shot, in adult female griffon vultures found in the Iberian Peninsula. The birds were found prostrate and immediately transferred to a wildlife rehabilitation center, where they died within 24 hours after supportive treatment. Necropsy and histopathologic examinations were done in 2 birds and metal analyses were done in all birds to determine the birds' causes of death. In one vulture, 9 uneroded lead pellets were recovered from the stomach, and moderate to severe hemosiderosis was seen histologically in the liver, lungs, and kidneys. Diagnosis of lead poisoning was confirmed by results of metal analyses, which revealed extremely high lead concentrations in blood (969-1384 μg/dL), liver (309-1077 μg/g dry weight), and kidneys (36-100 μg/g dry weight) for all 3 vultures. To prevent lead poisoning in vultures and preserve their populations in the Iberian Peninsula, more resources are needed for diagnosis and treatment of wildlife in rehabilitation centers, new regulations enabling the abandonment of fallen stock in the field must be approved, and lead ammunition must be prohibited in big-game hunting.

Global Positioning System (GPS) tracking offers key information in the study of movement ecology of threatened species. Nevertheless, the placement of GPS devices requires animal capture and handling, which may represent a challenge to the individual’s survival after release, mainly due to capture myopathy. The Pyrenean Capercaillie (Tetrao urogallus aquitanicus) is a threatened galliform especially sensitive to handling, extremely elusive, and challenging to capture. Our goal was to adapt a sedation protocol for Pyrenean Capercaillies undergoing GPS tagging, in order to increase their welfare and safety during the procedure. From 2018 to 2021, 23 wild Pyrenean Capercaillies were captured and sedated for GPS tagging as part of a European conservation project of emblematic Pyrenean avian species. The birds received intramuscular (IM) sedation with midazolam (ranging from 1.9 mg/kg to 8.08 mg/kg) and were handled for 20 to 40 min. Sedation was reversed with flumazenil (0.1 mg/mL IM). The sedated capercaillies were less responsive to stimuli (i.e., closed eyes and recumbency), showing discrete to no response to handling (i.e., placement of the GPS device, physical examination, cloacal temperature measurement, or reflex tests). Such response was compared in birds with sedation doses above and below the average dose (5.17 mg/kg). Only one clinical sign showed statistically significant differences between the two groups (“open-mouth breathing” sign, p = 0.02). A mortality rate of 4.35% was registered (one individual died during handling). Sedation facilitated the handling of the birds and faster interventions in the field, without increasing mortality when compared to handling without sedation. Therefore, sedation was shown to be a useful tool to reduce stress related to capture and handling of the threatened Pyrenean Capercaillie.