An issue that is often debated in forensic traumatology is the differential diagnosis of hemorrhages into the human brain substance (HBS) of traumatic and non-traumatic genesis. Objectives. This study aims to identify new criteria for objective forensic differentiation of hemorrhages of traumatic origin, cerebral infarction of ischemic and hemorrhagic genesis by using the method of complex degree of mutual anisotropy. For this study native sections of HBS from 125 corpses were used in the case of: death from coronary heart disease - 35 (28%) of native sections (Group 1 - control); hemorrhages of traumatic genesis - 30 (24%) sections (Group 2); ischemic cerebral infarction - 30 (24%) native sections(Group 3); and hemorrhages of non-traumatic genesis - 30 (24%) native sections (Group 4). Results. The statistical moments of the third and fourth orders, which characterize the asymmetry and excess of the complex degree of mutual anisotropy module size distributions, the strength of the method of polarization-correlation microscopy in the differentiation of the samples of the histological sections of the brain of control and experimental groups reached a good level — 87%-90%. Conclusion. The method of complex degree of mutual anisotropy allows differentiating with great precision the genesis of hemorrhage into the substance of the brain.
Abstract:Introduction: Investigation of the rheological properties of the erythrocytes is one of the crucial issues in the pathogenesis of most diseases of internal organs. Changes in the morphological structure of the red blood cell membrane serve as an early diagnostic criterion of coronary artery disease, diabetes mellitus type 2 and anaemia, thus a search for modern methods of investigation which can be used for early detection of erythrocytes membrane disorders is of great importance. Methods of laser polarimetry of the erythrocytes suspension smear are crucial in this area. Objectives: Investigation of the possible structural changes of the erythrocytes membranes in patients with coronary artery disease, diabetes mellitus type 2 and anaemia of different degrees of severity. Methods: For the objective assessment of the structural state of erythrocytes membrane laser polarimetry of the red cell suspension smear was applied. Set of the statistical points of the 1 st -4 th grades was calculated and their effectiveness in the early pre-clinical detection of the erythrocytes membrane disorder was established. Results: Images of the erythrocytes suspension samples, registered in the co-axial and crossed planes of the polarizer and analyser transmissions, regardless of the investigated group, are coordinating heterogeneous. However, obvious signs of the changes of the images structure depending on the presence and type of pathology are not visually detected. As the basis of an objective approach, a statistical analysis with histograms of the coordinate distributions of the random variables and a statistical calculation of the statistical points of the 1 st -4 th grades should be used. All statistical points, which describe the coordinate distributions of the various parameters of the Stokes vector, have individual sets of values and may be used for the differential diagnosis of the coronary artery disease, diabetes mellitus type 2 and anaemia. Conclusions: Interconnections between the values of the sets of the statistical points of the 1 st -4 th grades, which characterized coordinate distributions of the intensity, azimuth and ellipticity of the polarization of the erythrocytes suspension laser images of the elderly and senile patients with comorbidity were established by means of laser polarimetry. The most sensitive were asymmetry and kurtosis, which can be used for early detection of the erythrocytes membrane disorder when routine methods of its visualization and estimation still are not able to be applied.
In a review article current data of cerebrospinal fluid investigation methods are present. It is described their ability to solve urgent issues of forensic practice, namely the postmortem interval, time of the injuries appearence and diagnosis of pathological states and processes that preceded or caused the death. In particular, the possibility of biochemical analytical methods of changes diagnosis of CSF compared with changes in biochemical parameters in the human blood both before and after death. Indicators studied spectrophotometric investigation of cerebrospinal fluid obtained by the authors as the people who died as a result of unexpected death, and drug overdose. Particular attention was paid to biophysical research methods because, as at present relatively little studied, they are promising, informative, relatively simpleand inexpensive when they are used in the of forensic bureau practice. The application of liquor impedansometry research methods for establishing of intravitality occurrence injuries, their severity and duration of the process of dying is successful. Several authors have demonstrated the advantages and possibilities of using crystallographic methods in the cases of the death of various origins.It allows us to estimate the cause of death and postmortem interval. It was also stated on the effectiveness of this method in the diagnoses of intravitality of hanging. After analysis of the published data, we concluded that, unlike commonly used in forensic practice methods that are not always effective and may not be reliable, modern investigation abilities liquid media and body fluids by laser polarimetry will identify and implement a practice new optical criteria and to develop new methods to diagnose postmortem interval and intravitality of the injuries.
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