Objective: To elicit the structure of isolated compounds from roots of sidaguri (Sida rhombifolia Linn). Material and Methods: Several organic standard protocols were involved, including extraction, fractionation, and phytochemical testing. Further spectroscopy methods, FTIR and 1HNMR, were used to determine the predicted structure of molecules, while their ability to inhibit cyclooxygenase (COX 1 and 2) were tested using in vitro method. Results: Overall assessments showed that the structure of the sidaguri is a long chain aliphatic carboxylic acid and identified as Z-3, 6, 6 trimethylhept-2-en-1-ol (T12) and nonanoic (T13). Both isolates significantly inhibit COX-1 and COX-2 non-selectively (the COX-1/COX-2 ratio for T12 was 0.91 and 0.82; while COX-1/COX-2 ratio for T13 was 0.89 and 0.87 at concentrations of 0.05 and 0.025 µg/mL respectively). Conclusion: The active compounds of Sidaguri have antiinflammatory effect by inhibiting COX non-selectively.
Background: Arsenic paste was used to devitalize dental pulp in some problems before surgical, but it has been proven to have many hazardous effects. So, other alternative drugs are needed that are equally effective but safer. Jatropha (Jatropha curcas L.) can cause lysis blood vessels in the pulp, whereas the sidaguri (Sidarhombifolia L.) are effective in relieving inflammation. The purpose of this study was to determine the devitalization effect of jatropha and sidaguri on rabbit animal model.Method: Sidaguri roots were extracted using reflux method while jatropha sap was dried using lyophilization method. For easy application, the extract made into a paste (1; 1) and inserted into the cavity. After 7 days of exposure, the teeth were removed, calcified and followed to histopathological staining and COX-2 expression (IHC).Result: Histopathological examination showed that the paste was able to cause necrosis of pulp nerve. Higher dose of the drug increased the necrosis area, but the paste is not able to reduce COX-2 expression.Conclusion: It can be concluded that the paste has a potential to developed as devitalization agent although it is necessary to study to the reduce the inflammation process.
One disease that often affects the pulp is necrotic teeth, which are caused by some irritants. Most major irritants aremicrobes, such as bacteria. This study aimed to determine the type of bacteria in the necrotic root canal. Samples wereobtained from fifteen patients with necrotic teeth in Conservation Clinic of Dental Hospital, Faculty of Dentistry,Hasanuddin University. Specimens of bacteria from samples taken using paper points were inserted into the root canal.The specimens identified in the Laboratory of Microbiology, Faculty of Medicine. The results showed that there areseven types of bacteria that vary in number, most are gram-positive facultative anaerobic bacteria Actinomyces spp. Itwas concluded that Actinomyces spp. is the dominant species of bacteria on the necrotic tooth root canal.
This study examined the effect of tannic acid solution on dissolution of dentine collagen and morphological aspects of tendon collagen. Using root dentine, which was cut off from bovine anterior tooth, dentine powders were obtained by the pulverization and lyophilization. They were subject to an application of 1, 3, 5 or 10% tannic acid (TA) solution for 1, 3, 6, 12 or 24 h. TA-treated dentine powders were treated with 40% phosphoric acid (PA) for 30 s at 20 degrees C and additionally with trypsin. Released hydroxyproline in Woessner's assay after a hydrolysis in 6 N HCl at 110 degrees C for 20 h was assumed to be dissolved dentine collagen. Released hydroxyproline in a control sample without acid treatment decreased from 100 to about 60% with increased TA concentration of 1 to 10%, and decreased with increased incubation times of 1 to 24 h when applied by 5% TA solution. Scanning electron microscopy results established the morphological effect of their surface characteristics due to such treatments as 40% PA for 30 s and 5% TA for 6 h, or 40% PA after 5% TA treatment, yielding collagen structures protected by TA to attack from phosphoric acid.
Objective:The purpose of this study was to evaluate the effect of herbal alternatives, extract of sidaguri (sida rhombifolia) combine with extract of jatropha sap (jatropha curcas) application on microhardness and morphology of tooth surface. Material and Methods: Twelve upper first premolar teeth were used in this study, prepared to Class I box cavities, and were then allocated into 4 groups (n=3). Group 1-3 were treated groups, applied with combined extracts of a small dose (5 mg), medium dose (25 mg), and high dose (50 mg), while group 4 was the control group. The cavities were restored with composite resin, immersed in artificial saliva, and stored at room temperature for 14 days. The fillings and extracts were removed and cleaned under running tap water. The teeth were cut in a vertical direction towards the apex, sectioned into 1 mm thickness, and evaluated using Laser Scanning Microscope (LSM), then were examined for microhardness test. Results: All treated groups showed significantly lower microhardness than the control group. Tooth porosity was observed, and its extensiveness was affected by doses of extracts. Conclusion:The application of herbal alternatives (extract of sidaguri and jatropha) lowered the microhardness of tooth structure and caused tooth porosities.
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