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Abstract

This study examined the effect of tannic acid solution on dissolution of dentine collagen and morphological aspects of tendon collagen. Using root dentine, which was cut off from bovine anterior tooth, dentine powders were obtained by the pulverization and lyophilization. They were subject to an application of 1, 3, 5 or 10% tannic acid (TA) solution for 1, 3, 6, 12 or 24 h. TA-treated dentine powders were treated with 40% phosphoric acid (PA) for 30 s at 20 degrees C and additionally with trypsin. Released hydroxyproline in Woessner's assay after a hydrolysis in 6 N HCl at 110 degrees C for 20 h was assumed to be dissolved dentine collagen. Released hydroxyproline in a control sample without acid treatment decreased from 100 to about 60% with increased TA concentration of 1 to 10%, and decreased with increased incubation times of 1 to 24 h when applied by 5% TA solution. Scanning electron microscopy results established the morphological effect of their surface characteristics due to such treatments as 40% PA for 30 s and 5% TA for 6 h, or 40% PA after 5% TA treatment, yielding collagen structures protected by TA to attack from phosphoric acid.

Investigation on interaction of tannic acid with type I collagen and its effect on thermal, enzymatic, and conformational stability for tissue engineering applications

“…TA is one of the most popular leather tanning agents, which is present in a wide variety of plants. And its prevalence in more recent medical literature has been in the areas of wound healing, dental restoration, bioprosthetic heart valve replacement, etc . Traditionally TA was used as a standard topical agent in treatment of burn wound to fix the denatured matrix of damaged skin in an effort to minimize pain, infection, and healing time but this practice was discontinued due to suspected issues of hepatotoxicity.…”

“…Traditionally TA was used as a standard topical agent in treatment of burn wound to fix the denatured matrix of damaged skin in an effort to minimize pain, infection, and healing time but this practice was discontinued due to suspected issues of hepatotoxicity. However, improvements made in the TA extraction, separation and purification technologies has reduced the issue of hepatotoxicity been studied extensively as a possible candidate for antitumor therapies and have a number of biomedical applications . Association of TA with biopolymers such as gelatin, albumin and some polysaccharides through noncovalent interactions are well established .…”

Chitosan/collagen blends with inorganic and organic additive—A review

“…[134,135] Tannic acid was used for many decades in the treatment of burns. [136][137][138][139] In biomaterial science, it was studied as a collagen [140][141][142][143][144][145][146][147][148][149][150][151][152] and chitosan, [153][154][155][156][157][158][159][160] as well as their mixture [161] cross-linker. Tannic acid has antioxidant and antimicrobial properties.…”

“…Tannic acid was used for many decades in the treatment of burns . In biomaterial science, it was studied as a collagen and chitosan, as well as their mixture cross‐linker. Tannic acid has antioxidant and antimicrobial properties .…”