Irrigation of root canal is an essential part that supports the success of root canaltreatment as it removes necrotic tissues, microorganisms and dentin chips frominfected root canal through flushing of irrigants. Despite its usefulness as anantimicrobial agent, irrigants are also toxic that can cause irritations wheninadvertently forced into the periapical tissue. The choice of irrigants needs goodknowledge of irrigants’ characteristics, undertsanding an appropriate technique ofirrigation, and type of microorganisms involved in root canal infection that supportthe effectiveness of irrigants. This paper is aimed to discuss the characteristics ofsome irrigants commonly used in root canal treatment and also to present the resultsobtained from some clinical investigations.
Objective: To elicit the structure of isolated compounds from roots of sidaguri (Sida rhombifolia Linn). Material and Methods: Several organic standard protocols were involved, including extraction, fractionation, and phytochemical testing. Further spectroscopy methods, FTIR and 1HNMR, were used to determine the predicted structure of molecules, while their ability to inhibit cyclooxygenase (COX 1 and 2) were tested using in vitro method. Results: Overall assessments showed that the structure of the sidaguri is a long chain aliphatic carboxylic acid and identified as Z-3, 6, 6 trimethylhept-2-en-1-ol (T12) and nonanoic (T13). Both isolates significantly inhibit COX-1 and COX-2 non-selectively (the COX-1/COX-2 ratio for T12 was 0.91 and 0.82; while COX-1/COX-2 ratio for T13 was 0.89 and 0.87 at concentrations of 0.05 and 0.025 µg/mL respectively). Conclusion: The active compounds of Sidaguri have antiinflammatory effect by inhibiting COX non-selectively.
According to 2009 Indonesian Health Profile Data, pulp and periapical diseases were 8thof the 10 outpatients athospital. This situation tends to increase become the big of 7. Pulp and periapical diseases occur due to bacterialinfection in the pulp tissue. The bacteria are often found in the root canal, namely E. faecalis, and Actinomyces spp.Various herbs commonly used by people to treat dental diseases, i.e. root sidaguri. However, sidaguri plants have notbeen studied in the field of dentistry as an antibacterial. This study aimed to determine the effects of root sidaguriagainst E. faecalis and Actinomyces spp. Inhibition test of ethanol extract of the roots sidaguri against E. faecalis andActinomyces spp. using agar diffusion method at concentrations of 5%, 10%, 15%, and 20% based on themeasurement of inhibition zone by each concentration. The greatest inhibition against E. faecalis was performed by concentration of 20% (p<0.05), while no inhibition zone was found on Actinomyces spp. It was concluded that theethanol extract of the sidaguri roots was the most effective against E. faecalis at concentration of 20%, but not effectiveat all against Actinomyces spp.
Background: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing and anti-inflammation. This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. Materials and Methods: Firstly, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by MTT assay on 24 h after the last treatment. While cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 µg/mL). Untreated cells were used as control. Result: Adding of HFE at range 25.0 till 100 µg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p<0.05). Conclusion: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent. BACKGROUND: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing, and anti-inflammation. AIM: This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. MATERIALS AND METHODS: First, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay on 24 h after the last treatment, while cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 μg/mL). Untreated cells were used as control. RESULTS: Adding of HFE at range 25.0 until 100 μg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p < 0.05). CONCLUSION: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent.
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