Objective: To examine the expression of caspase-3 and IL-1β following application of Pulp Out® on rabbit's pulp teeth as well as histopathology. Material and Methods:The study was conducted on the maxillary incisors rabbits. The teeth were prepared and Pulp Out® was inserted at the base of prepared cavity, then restored with RM-GIC. After 24 hours, the animals were then euthanized and processed for histopathology and immunohistochemistry evaluation. Results:Our results indicated that Pulp Out®, when administered to the pulp teeth, increased the number and diameter of blood vessels. The expression of caspase-3 and IL-1β showed similarities to the expression of commercial devitalizing agent. Conclusion: Our results revealed that Pulp Out® is a valuable devitalization agent that should be further explored for its safety before clinical application.
This study aims to determine the characteristics of the microhardness impairment root canal dentin after application with different types of EDTA. Samples mandibular premolar teeth with one root canal, each divided into 4 groups: EDTA solution, EDTA gel, EDTA cream and negative control; and each group consisted of 6 samples. The teeth were decoronated at cementoenamel junction (CEJ), prepared by the crown down pressureless technique, cut along longitudinal direction, and each sample was attached to selfcured acrylic and then soaked in distilled water. Samples were taken early microhardness measurement by means of Digital Vickers Microhardness Tester. The sample is then applied to the appropriate group of materials EDTA for 5 minutes, except for the negative control group, soaked in saline solution for 5 menit, then performed the final measurement of microhardness of dentin. The results of measurements taken from the average value of measurements made at 3 points, coronal, middle and apical. Data were collected and analyzed using ANOVA and Tukey’s Post Hoc test.The results showed there are differences in dentin microhardness decrease significantly in all treatment groups compared to the negative control group (p <0.05) with the largest drop in the group EDTA solution, amounting to 13 667 kg / mm2. Nevertheless, based on the results of different statistical test further, discovered the value of p> 0.05 which means there is no difference in microhardness reduction in dentin significantly among the test group.
<p>This study was aimed to determine the effect of epoxy resin-based and MTA-based sealer on fiber post bond strength in the root canal wall.Samples are mandibulary first premolar, with a single root canal. They are divided into three groups: negative control group which is a gutta percha obturation without sealer, gutta percha obturation with epoxy resin based sealer [AH Plus], and gutta percha obturation with MTA-based sealer [MTA Fillapex] groups. Samples were decoronated, prepared, obturated and then stored in the incubator at room temperature for one week, the post space were prepared for fiber post insertion. Samples were mounted in the PVC pipes before insertion. Samples were stored in the incubator for one day before bond strength testing. Universal testing machine was used with the speed of 0,5 mm/minutes. Data were collected and analysed using ANOVA.The result showed that the fiber post bond strength in the root canal obturated with epoxy resin-based sealer was higher [12.311 N/mm<sup>2</sup>] than MTA-based sealer [10.786 N/mm<sup>2</sup>], but that result was not statistically significant. Therefore, it was concluded that the root canal obturated with epoxy resin-based sealer did not yield a significant bond strength [p = 0.689] compared to MTA-based sealer [p> 0.05].</p>
Structure of the anterior teeth after endodontic treatment usually become weak because of the extensive loss of toothstructure. It causes a big problem to an endodontically treatment tooth when considering its restoration, examplereduced strength of the remaining tooth structure. Restoration that covers the tooth crown can be used if aesthetic andfunctional problems factors have been considered. However, composite resins are also often the treatment of choice forthe restoration of endodontically treated tooth. In this literature review, it will be discussed the selection of the properrestoration of the anterior teeth that have been endodontically treated.
Background: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing and anti-inflammation. This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. Materials and Methods: Firstly, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by MTT assay on 24 h after the last treatment. While cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 µg/mL). Untreated cells were used as control. Result: Adding of HFE at range 25.0 till 100 µg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p<0.05). Conclusion: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent. BACKGROUND: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing, and anti-inflammation. AIM: This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. MATERIALS AND METHODS: First, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay on 24 h after the last treatment, while cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 μg/mL). Untreated cells were used as control. RESULTS: Adding of HFE at range 25.0 until 100 μg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p < 0.05). CONCLUSION: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent.
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