Commercial blueberry production has been a viable industry throughout the world for 95 years; because of blueberry is a good source of antioxidant. Blueberries are especially rich in anthocyanin, a flavonoid with potent antioxidant capacity. The aim of this study was to compare the phenolic quantities, antioxidant activities, anthocyanin, sugar and phenolic compounds of blueberries produced in Turkey with those of similar blueberry varieties produced around the world. As a result of the conducted analysis, the total phenolic content (TPC) amount found in the berries was 77.26-215.12 mg GAE/100 g, the total flavonoid content (TFC) was 30.44-91.69 mg QE/100 g and the total anthocyanin content (TAC) was 43.03-295.06 mg c3-GE/100 g. Examining the antioxidant activities of the berries, DPPH between 1.10-5.65 mg/ml, FRAP between 454.93-36832.96 µmol troloks/100 g, β-Carotene between 40.66-86.48%. It was determined that the natural berries contained much more phenolic compounds and higher antioxidant activity than that of the cultivars The result of HPLC analysis, chlorogenic acid is determined to be the dominant compound in all berries. Furthermore, fructose and glucose are found in all fruits in different quantities while sucrose is found in certain varieties of berries as well. At the end of the performed study the data indicate that wild and cultivars of blueberries are rich sources of antioxidants for local as well international industries importing this fruit for food processing and enormous products.
The gene encoding an alpha-L: -arabinofuranosidase from Geobacillus caldoxylolyticus TK4, AbfATK4, was isolated, cloned, and sequenced. The deduced protein had a molecular mass of about 58 kDa, and analysis of its amino acid sequence revealed significant homology and conservation of different catalytic residues with alpha-L: -arabinofuranosidases belonging to family 51 of the glycoside hydrolases. A histidine tag was introduced at the N-terminal end of AbfATK4, and the recombinant protein was expressed in Escherichia coli BL21, under control of isopropyl-beta-D-thiogalactopyranoside-inducible T7 promoter. The enzyme was purified by nickel affinity chromatography. The molecular mass of the native protein, as determined by gel filtration, was about 236 kDa, suggesting a homotetrameric structure. AbfATK4 was active at a broad pH range (pH 5.0-10.0) and at a broad temperature range (40-85 degrees C), and it had an optimum pH of 6.0 and an optimum temperature of 75-80 degrees C. The enzyme was more thermostable than previously described arabinofuranosidases and did not lose any activity after 48 h incubation at 70 degrees C. The protein exhibited a high level of activity with p-nitrophenyl-alpha-L: -arabinofuranoside, with apparent K (m) and V (max) values of 0.17 mM and 588.2 U/mg, respectively. AbfATK4 also exhibited a low level of activity with p-nitrophenyl-beta-D: -xylopyranoside, with apparent K (m) and V (max) values of 1.57 mM and 151.5 U/mg, respectively. AbfATK4 released L: -arabinose only from arabinan and arabinooligosaccharides. No endoarabinanase activity was detected. These findings suggest that AbfATK4 is an exo-acting enzyme.
Design and synthesis of a new type of bischalcones as an alternative to natural and synthetic bischalcones are reported for the first time. Key steps involved the solvent-free Claisen-Schmidt condensation of chalcones, and the successful first application of the diazotization-diazocoupling reaction in the synthesis of CNNC-linked bischalcones by simple structural modification of p-aminoacetophenone. The structures of all compounds were confirmed by means of FT-IR, (1) H and (13) C NMR, ESI/MS, and elemental analysis. In addition, the newly synthesized compounds were screened for carbonic anhydrase inhibition activities. Almost all bischalcones exhibited moderate-to-good inhibitory activities.
Imatinib, an Abelson (ABL) tyrosine kinase inhibitor, is a lead molecular-targeted drug against chronic myelogenous leukemia (CML). To overcome its resistance and adverse effects, new inhibitors of ABL kinase are needed. Our previous study showed that the benzyl ester of gypsogenin (1c), a pentacyclic triterpene, has anti-ABL kinase and a subsequent anti-CML activity. To optimize its activities, benzyl esters of carefully selected triterpenes (PT1–PT6), from different classes comprising oleanane, ursane and lupane, and new substituted benzyl esters of gypsogenin (GP1–GP5) were synthesized. All of the synthesized compounds were purified and charachterized by different spectroscopic methods. Cytotoxicity of the parent triterpenes and the synthesized compounds against CML cell line K562 was examined; revealing three promising compounds PT5, GP2 and GP5 (IC50 5.46, 4.78 and 3.19 μM, respectively). These compounds were shown to inhibit extracellular signal-regulated kinase (ERK) downstream signaling, and induce apoptosis in K562 cells. Among them, PT5 was identified to have in vitro activity (IC50 = 1.44 μM) against ABL1 kinase, about sixfold of 1c, which was justified by molecular docking. The in vitro activities of GP2 and GP5 are less than PT5, hence they were supposed to possess other more mechanisms of cytotoxicity. In general, our design and derivatizations resulted in enhancing the activity against ABL1 kinase and CML cells.
The composition of the essential oils obtained from the air-dried Teucrium chamaedrys L. subsp. chamaedrys, Teucrium orientale L. var. puberulens, and Teucrium chamaedrys L. subsp. lydium (Lamiaceae) were analyzed by GC-MS. Thirty-six, 35, and 33 components were identified in the essential oils, and germacrene D (16.7%) was the most abundant constituent in T. chamaedrys subsp. chamaedrys, and b-caryophyllene was the most abundant component in both T. orientale var. puberulens and T. chamaedrys subsp. lydium in the ratios 21.7% and 19.7%, respectively. The isolated essential oils of the plants were tested for antimicrobial activity and showed moderate antibacterial activity against Gram-positive and Gram-negative bacteria, but no antifungal activity was observed against two yeast-like fungi.
The structures of a new glyceryl phosphoryl ether [1] and an isocaffeine derivative (4) from the sea cucumber, Cucumaria frondosa, were elucidated by spectroscopic means and chemical transformations. Compound 1 is l-0-[(llZ)-octadecenyl]-m-glycery 1-3phosphorylcholine and compound 4 is 1,3,9-trimethyl-8-nitrasoisoxanthine. The dinucleotides thymidylyl-(3'-5')-thymidine [7], 2'-deoxyadenylyl-(3'-5')-thymidine [8] and thymidylyl-(3 '-5 ')-2 '-deoxyadenosine-3 '-monophosphate [93, in the form of their sodium salts, were also isolated from this same source.Recently we reported the isolation of novel sulfated hydrocarbons (1) and sulfated oligosaccharides (2) from the sea cucumber Cucumaria frondosa Gunnerus (Class Holothuroidea). In our continuing investigation of the polar constituents of this organism, we have encountered a novel glyceryl phosphoryl ether, 1, an isocaffeine derivative, 4, and three dinucleotides, 7, 8, and 9•The "H-nmr spectrum (pyridine-5/D2G) of compound 1 closely resembles those of known glyceryl phosphoryl ethers. The chemical shift assignments (Table 1) were facilitated by comparison with literature data (3-5) and reference to the 2D COSY nmr spectrum. The 15N-nmr spectrum shows a signal at 0.8 ppm confirming the presence of a nitrogen atom in the molecule (6). The 13C-nmr chemical shift assignments were made on the basis of DEPT and HETCOR spectra and comparison with data from known homologs (3-6). Thus, apart from the location of the double bond and the precise length of the side-chain, the structure of compound 1 was well defined by nmr methods.The positive fabms (glycerol) of 1 displayed a substantial ion at m/z 508 corresponding to M (C26H54N06P)+H, while significant fragments at m/z 256 and 166 are readily accounted for via fission at the ethereal bonds of the hydrocarbon and phosphoryl choline side-chains, respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.