Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%).
<p class="MDPI17abstract"><strong><span lang="EN-US">Tujuan:</span></strong><span lang="EN-US"> Mastitis merupakan suatu peradangan yang terjadi pada glandula intramamari sebagai respon dari mekanisme pertahanan tubuh. Kejadian dan tingkat infeksi mastitis sangat dipengaruhi oleh berbagai macam faktor penyebab. Penelitian ini bertujuan untuk mengidentifikasi faktor-faktor resiko yang berhubungan dengan kejadian mastitis subklinis pada kambing peranakan etawah (PE) di Kecamatan Kokap, Kulonprogo, Yogyakarta.</span></p><p class="MDPI17abstract"><strong><span lang="EN-US">Metode:</span></strong><span lang="EN-US">Jenis metode yang digunakan pada penelitian ini adalah metode survei. Teknik sampling yang digunakan dalam penelitian ini adalah <em>purposive sampling</em> dengan jumlah 200 sampel susu segar dari kambing PE. Pengumpulan data dilakukan dengan metode wawancara (kuesioner), pengamatan langsung dan pengujian status mastitis dengan menggunakan reagen <em>California Mastitis Test </em>(CMT). Data penelitan ini dianalisis secara diskriptif dan analisis <em>bivariat chi square</em> (X<sup>2</sup>), <em>Odds </em></span><em><span lang="EN-US">Ratio </span></em><span lang="EN-US">(OR), serta <em>Relative risk</em> (RR).</span></p><p class="MDPI17abstract"><strong><span lang="EN-US">Hasil: </span></strong><span lang="EN-US">Hasil</span><span lang="EN-US">penelitian</span><span lang="EN-US"> menunjukan bahwa faktor resiko yang berperan terhadap kejadian mastitis subklinis di Kecamatan Kokap</span><span lang="IN"> adalah</span><span lang="EN-US"> variabel umur sapih <2 bulan (X<sup>2</sup>=22,99; OR=21,13 dan RR=13,2) dan jarak pembuangan limbah <15 m dari kandang (X<sup>2</sup>=3,98; OR=</span><span lang="IN">6</span><span lang="EN-US">,</span><span lang="IN">52</span><span lang="EN-US"> dan RR=</span><span lang="IN">5</span><span lang="EN-US">,4</span><span lang="IN">4</span><span lang="EN-US">).</span></p><p><strong><span lang="EN-US">Kesimpulan: </span></strong><span lang="EN-US">Variabel faktor resiko umur sapih <2 bulan dan jarak pembuangan limbah <15 m dari kandang memiliki asosiasi kuat dengan infeksi mastitis subklinis di Kecamatan Kokap, Kulonprogo, Yogyakarta.</span></p>
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