Amalina N, Suyatmi, Suparyanti EL. 2010. Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis. Biofarmasi 8: 47-51. This research aimed to determine the effect of beluntas (Pluchea indica L.) leaf extract in habiting spermatogenesis. Flavonoids can inhibit aromatase enzyme that function to catalyze the conversion of androgens into estrogen so that the level of the testosterone hormone will increase. The high concentration of testosterone will affect on the feedback to the pituitary, that not releasing FSH and LH, so it will inhibit spermatogenesis. This research is an experimental research with post-test only controlled group design method, using male mice, 2-3 months age, weight 20-30 g, as many as 24 mice divided into 4 groups. The first group was a control group, the second group as treatment group I that given with 1.4 mg/20 g body weight beluntas extract, the third group as treatment group II that given with 2.8 mg/g body weight beluntas extract. The fourth group as treatment group III that given with 5.6 mg/g body weight beluntas extract. The treatment was given for 10 days after that the testes of mice were taken and from each testicle was made 3 slices and from each slice the most representative seminiferous tubule was taken for spermatid cells. So, each mice had 18 units of data would be analyzed. The data obtained were analyzed with Anova to determine the significant differences before and after the treatment of extract and to be compared the difference between four groups with Dunnet T3 test to determine the difference between each group. Based on the statistical test results with Dunnet T3, it showed a significant difference between the fours study groups, except between treatment groups I and II. This might be due to the effectiveness of the two doses equally so that by doubling the dose, it did not give a doubling effect on decreasing spermatids. The provision of beluntas leaf extract can cause a decrease in the number of spermatid cells in mice. The average spermatid cells number decreased with increasing beluntas leaf extract dose. The best dose for reducing the number of spermatids in this study was the dose in the treatment group III that was 5.6 mg/g body weight.
Myzus persicae is the main pest in chili which causes decreasing production and qualities of chili. The was aimed to know the effect of Pomelo and Orange’s peel extract to controlling green peach aphids population. The research was conducted in screen house located in Colomadu, Karanganyar regency from October 2017- January 2018. This research used Randomized Complete Design with 2 factors i.e variety of orange’s peel extract and levels of concentration. The various Citrus’s peel extract consisted of Pomelo’s peel extract and Orange’s peel extract. Levels of concentration from each extracts i.e 1%, 2%, 4%, 8%, and 16%. Each treatment replicated 5 times. The result showed that Citrus peel extracts had effect on the green peach aphid population and had no effect on the growth of chili. The LC50 value of Pomelo and Orange’s peel extracts were 4,77% and 5,56%. Pomelo and orange peel extract treatment on differrent concentration was not give effect on the percentage of the attack intensity.
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