Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis

Abstract

Amalina N, Suyatmi, Suparyanti EL. 2010. Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis. Biofarmasi 8: 47-51. This research aimed to determine the effect of beluntas (Pluchea indica L.) leaf extract in habiting spermatogenesis. Flavonoids can inhibit aromatase enzyme that function to catalyze the conversion of androgens into estrogen so that the level of the testosterone hormone will increase. The high concentration of testosterone will affect on the feedback to the pituitary, that not releasing FSH and LH, so it will inhibit spermatogenesis. This research is an experimental research with post-test only controlled group design method, using male mice, 2-3 months age, weight 20-30 g, as many as 24 mice divided into 4 groups. The first group was a control group, the second group as treatment group I that given with 1.4 mg/20 g body weight beluntas extract, the third group as treatment group II that given with 2.8 mg/g body weight beluntas extract. The fourth group as treatment group III that given with 5.6 mg/g body weight beluntas extract. The treatment was given for 10 days after that the testes of mice were taken and from each testicle was made 3 slices and from each slice the most representative seminiferous tubule was taken for spermatid cells. So, each mice had 18 units of data would be analyzed. The data obtained were analyzed with Anova to determine the significant differences before and after the treatment of extract and to be compared the difference between four groups with Dunnet T3 test to determine the difference between each group. Based on the statistical test results with Dunnet T3, it showed a significant difference between the fours study groups, except between treatment groups I and II. This might be due to the effectiveness of the two doses equally so that by doubling the dose, it did not give a doubling effect on decreasing spermatids. The provision of beluntas leaf extract can cause a decrease in the number of spermatid cells in mice. The average spermatid cells number decreased with increasing beluntas leaf extract dose. The best dose for reducing the number of spermatids in this study was the dose in the treatment group III that was 5.6 mg/g body weight.

Phytoconstituents and Pharmacological Activities of Indian Camphorweed (Pluchea indica): A Multi-Potential Medicinal Plant of Nutritional and Ethnomedicinal Importance

“…Many plants and their constituents have been investigated for their antifertility potential [105]. Interestingly, P. indica has been used orally in men as an antifertility alternative medicine [20,21,106].…”

“…Spermiogenesis is the last phase of spermatogenesis in which spermatids are converted to spermatozoa, which play a substantial function in the fertilization process [107]. It was reported that the leaf extract (doses 1.4, 2.8, and 5.6 mg/kg for 10 days) caused a decrease in the number of spermatid cells in male mice when the dose increased [106]. It was found that the treatment of white male adult rats with leaf tannin reduced the number of spermatozoa and spermatids [20,21].…”

Lactogenic activity of ethanolic extract of Pluchea indica Less leaf in lactating rats

“…Pharmacological studies have found that P. indica inhibits spermatogenesis (6), induces apoptosis of nasopharynx cancer cells (7), has anti-inflammatory (8), antipyretic (9), antibacterial (10), antioxidant (11) and antidiabetic activities (12). P. indica produces secondary metabolites such as flavonoids, alkaloids, tannins, and saponins (13).…”

Curcumin, a potential oral herbal male contraceptive: a review article

“…Traditional medicinal plants with effects on infertility must be acceptable to the community, safe, productive, reversible, and not affect with sexual intercourse. 11, 12 Since natural medications were believed to be healthier than synthetic ones, interest in using medicinal herbs and traditional medicine increased, and it began mostly in the last decade.…”

“…Turmeric and other Indonesian medicinal herbs have also been shown to affect male antifertility possibly. [12][13][14][15][16] Curcuma longa and curcumin For thousands of years, natural products have been used in traditional medicine and have proven to be a promising source of components for new drug development. 14,15 Turmeric (Curcuma longa Linn) is cultivated worldwide in subtropical and tropical regions, stems from Indonesia, India, and Southeast Asia and belongs to the Zingiberaceae family.…”