Collagen, the major
structural component of nearly all mammalian
tissues, undergoes extensive proteolytic remodeling during developmental
states and a variety of life-threatening diseases such as cancer,
myocardial infarction, and fibrosis. While degraded collagen could
be an important marker of tissue damage, it is difficult to detect
and target using conventional tools. Here, we show that a designed
peptide (collagen hybridizing peptide: CHP), which specifically hybridizes
to the degraded, unfolded collagen chains, can be used to image degraded
collagen and inform tissue remodeling activity in various tissues:
labeled with 5-carboxyfluorescein and biotin, CHPs enabled direct
localization and quantification of collagen degradation in isolated
tissues within pathologic states ranging from osteoarthritis and myocardial
infarction to glomerulonephritis and pulmonary fibrosis, as well as
in normal tissues during developmental programs associated with embryonic
bone formation and skin aging. The results indicate the general correlation
between the level of collagen remodeling and the amount of denatured
collagen in tissue and show that the CHP probes can be used across
species and collagen types, providing a versatile tool for not only
pathology and developmental biology research but also histology-based
disease diagnosis, staging, and therapeutic screening. This study
lays the foundation for further testing CHP as a targeting moiety
for theranostic delivery in various animal models.
Senescence is a mechanism associated with aging that alters tissue regeneration by depleting the stem cell pool. Chronic obstructive pulmonary disease (COPD) displays hallmarks of senescence, including a diminished stem cell population. DNA damage from cigarette smoke (CS) induces senescence via the p16 pathway. This study evaluated the contribution of p16 to CS-associated lung pathologies. p16 expression was prominent in human COPD lungs compared with normal subjects. CS induces impaired pulmonary function, emphysema, and increased alveolar epithelial cell (AECII) senescence in wild-type mice, whereas CS-exposed p16
−/−
mice exhibit normal pulmonary function, reduced emphysema, diminished AECII senescence, and increased pro-growth IGF1 signaling, suggesting that improved lung function in p16
−/−
mice was due to increased alveolar progenitor cell proliferation. In conclusion, our study suggests that targeting senescence may facilitate alveolar regeneration in COPD emphysema by promoting IGF1 proliferative signaling.
Changes in anteroposterior diameters of the rib cage and abdomen are sensed with magnetometers and summed to give outputs which are very nearly linearly related to changes in lung volume. The volume events of breathing can be measured without recourse to a mouthpiece or face mask, other than for calibration, and with minimal encumbrance to the subject.
The crystal structure of the complex between the cross-reacting antigen Guinea fowl lysozyme and the Fab from monoclonal antibody F9.13.7, raised against hen egg lysozyme, has been determined by x-ray diffraction to 3-A resolution. The antibody interacts with exposed residues of an alpha-helix and surrounding loops adjacent to the lysozyme active site cleft. The epitope of lysozyme bound by antibody F9.13.7 overlaps almost completely with that bound by antibody HyHEL10; the same 12 residues of the antigen interact with the two antibodies. The antibodies, however, have different combining sites with no sequence homology at any of their complementarity-determining regions and show a dissimilar pattern of cross-reactivity with heterologous antigens. Side chain mobility of epitope residues contributes to confer steric and electrostatic complementarity to differently shaped combining sites, allowing functional mimicry to occur. The capacity of two antibodies that have different fine specificities to bind the same area of the antigen emphasizes the operational character of the definition of an antigenic determinant. This example demonstrates that degenerate binding of the same structural motif does not require the existence of sequence homology or other chemical similarities between the different binding sites.
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