3D printing technology has an enormous potential to apply to chemical engineering education. In this paper, we describe several designs of 3D printed mesoreactors (Y-shape, T-shape, and Long channel shape) using the following steps: reactor sketching, CAD modeling, and reactor printing. With a focus on continuous plug flow mesoreactors (PFRs, i.d. = 2 mm), fluid mixing phenomena were explored by using a passive mixing method. The small channel of mesoreactors facilitates the stability of a laminar flow in the system at low Reynolds number. With changes in flow rates (0.2 and 4.0 mL/min) and channel geometry (0° and 90° outlet angle), fluid mixing was controlled. Our results provided that 3D printed mesoreactors can be possibly used in teaching fluid dynamics, chemical kinetics, and reaction engineering, which are main courses of the chemical engineering undergraduate program. Furthermore, the cost of mesoreactor printing was suitable (<$1.00/microreactor) for education.
Observing biochemical processes within living cell is imperative for biological and medical research. Fluoresce imaging is widely used for intracellular sensing of cell membranes, nuclei, lysosomes, and pH. Electrochemical assays have been proposed as an alternative to fluorescence‐based assays because of excellent analytical features of electrochemical devices. Notably, thanks to the rapid progress of micro/nanotechnologies and electrochemical techniques, intracellular electrochemical sensing is making rapid progress, leading to a successful detection of intracellular components. Such insight can provide a deep understanding of cellular biological processes and, ultimately, define the human healthy and diseased states. In this review, we present an overview of recent research progress in intracellular electrochemical sensing. We focus on two main topics, electrochemical extraction of cytosolic contents from cells and intracellular electrochemical sensing in situ.
Bipolar electrochemistry has attracted great interest for applications based on sensing, electrografting, and electrodeposition, because the technique enables electrochemical reactions to be induced at multiple bipolar electrodes (BPEs) with only a single power supply. However, there are only a few reports on the biofabrication of hydrogels using BPEs. In this study, we applied bipolar electrochemistry to achieve the electrodeposition of calcium-alginate hydrogels at specified target areas, which is possible because of the use of water electrolysis to obtain acidification at the anodic pole. This scheme was used to successfully fabricate an array of hydrogel deposits at a BPE array. In addition, hydrogels were successfully fabricated either at only the target BPEs or only the target areas of BPEs by repositioning the driving electrodes. Furthermore, a hydrogel was drawn on a large BPE as a canvas by using small driving electrodes. As a demonstration of the electrodeposited hydrogels for bioapplications, mammal cells were cultured in the hydrogels. Because the amount and shape of the hydrogel deposits can be controlled by using the bipolar system, the system we developed can be used for biosensors and cell culture platforms.
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