Intracellular Electrochemical Sensing

Ino, Kosuke; Nashimoto, Yuji; Taira, Noriko; Ramón‐Azcón, Javier; Shiku, Hitoshi

doi:10.1002/elan.201800410

Cited by 23 publications

(24 citation statements)

References 132 publications

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“…We previously reviewed the progress in intracellular electrochemical sensing techniques (Ino et al, 2018b). Here, we focus on two main types of electric and electrochemical microfluidic devices for lysing cells and collecting components of cells by applying pulse voltage.…”

Section: Collection Of Subcellular Cytoplasmmentioning

confidence: 99%

“…In particular, large-scale integration (LSI) technology will be used for incorporating many addressable electrodes into microfluidic devices. Indeed, LSI devices have already been used for electrochemical imaging (Inoue et al, 2012, 2015; Sen et al, 2013a, 2014; Abe et al, 2015, 2016; Kanno et al, 2015a,b, 2016, 2017; Ino et al, 2017, 2018b,c,d, 2019a,b). Since signal amplifiers and switching elements are incorporated into the sensing area, highly sensitive sensors can be incorporated with high density.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

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Electric and Electrochemical Microfluidic Devices for Cell Analysis

et al. 2019

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Microfluidic devices are widely used for cell analysis, including applications for single-cell analysis, healthcare, environmental monitoring, and organs-on-a-chip that mimic organs in microfluidics. Moreover, to enable high-throughput cell analysis, real-time monitoring, and non-invasive cell assays, electric and electrochemical systems have been incorporated into microfluidic devices. In this mini-review, we summarize recent advances in these systems, with applications from single cells to three-dimensional cultured cells and organs-on-a-chip. First, we summarize microfluidic devices combined with dielectrophoresis, electrophoresis, and electrowetting-on-a-dielectric for cell manipulation. Next, we review electric and electrochemical assays of cells to determine chemical section activity, and oxygen and glucose consumption activity, among other applications. In addition, we discuss recent devices designed for the electric and electrochemical collection of cell components from cells. Finally, we highlight the future directions of research in this field and their application prospects.

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Section: Collection Of Subcellular Cytoplasmmentioning

confidence: 99%

Section: Conclusion and Prospectsmentioning

confidence: 99%

Electric and Electrochemical Microfluidic Devices for Cell Analysis

et al. 2019

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“…Micro-to nanoscale electrochemical devices are widely used for bioanalysis, including cell analysis from single cells to threedimensional (3D) cultured cells, [1][2][3][4][5][6][7] although optical imaging is also commonly utilized for bioanalysis. 8 For example, a scanning single electrode was utilized for imaging cell activity because this electrochemical method can detect chemicals derived from cells.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Imaging of Cell Activity in Hydrogels Embedded in Grid-shaped Polycaprolactone Scaffolds Using a Large-scale Integration-based Amperometric Device

et al. 2018

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Tissue engineering requires analytical methods to monitor cell activity in hydrogels. Here, we present a method for the electrochemical imaging of cell activity in hydrogels embedded in printed polycaprolactone (PCL) scaffolds. Because a structure made of only hydrogel is fragile, PCL frameworks are used as a support material. A grid-shaped PCL was fabricated using an excluder printer. Photocured hydrogels containing cells were set at each grid hole, and cell activity was monitored using a large-scale integration-based amperometric device. The electrochemical device contains 400 microelectrodes for biomolecule detection, such as dissolved oxygen and enzymatic products. As proof of the concept, alkaline phosphatase and respiration activities of embryonic stem cells in the hydrogels were electrochemically monitored. The results indicate that the electrochemical imaging is useful for evaluating cells in printed scaffolds.

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“…For the probe scanning approach, various types of physical or chemical natures at surfaces were characterized in situ. Scanning electrochemical microscopy (SECM) [4][5][6][7][8][9][10][11][12][13] uses a microelectrode as a scanning probe to detecting redox current in localized areas in order to afford images based on the electrochemical properties of functional surfaces. The extracellular detection scheme shares its technical advantages and limitations with SECM.…”

Section: Introductionmentioning

confidence: 99%

“…We have developed various types of conical probes, including optical fiber-electrode, glass capillary-electrode, and double barrel probes. 5,16 For non-scanning electrochemical bio-devices, electrode arrays can be integrated on a cellular chip for high-throughput real time monitoring of biological functions. [17][18][19][20][21][22] Previously, we have reviewed progress of whole-cell biosensors, especially incorporation of microfabrication technologies and genemodified engineering.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensing System for Single Cells, Cellular Aggregates and Microenvironments

Shiku

2018

ANAL. SCI.

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Applications of electrochemical biosensing for surveying intact cells and tissues have been focus of attention. Two experimental approaches have been used when performing amperometric measurements on biological cells, the stylustype microelectrode probes and the electrode-integrated microdevices based on lithographic technologies. For the probe scanning approach, various types of microsensors were developed to monitor localized physical or chemical natures at a variety of surfaces in situ under wet conditions. Scanning electrochemical microscopy (SECM) has been applied for monitoring local oxygen, enzyme activity, and collection of transcripts. For the non-scanning type of approach, electrode array devices allow very rapid response, parallel monitoring, and multi-analyte assay. Sveral topics of on-chip-culture system were introduced especially concerning on gene expression monitoring by reporter system and reconstruction of in vivo-like nature by controlling microenvironments. Electrochemical reporter assay has been demonstrated to monitor the gene expression process of the gene-modified cultured cells. Long-term monitoring of cellular function of spheroids and three dimensionally-cultured cells were carried out by controlling microenvironments on the cellular chip.

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Intracellular Electrochemical Sensing

Cited by 23 publications

References 132 publications

Electric and Electrochemical Microfluidic Devices for Cell Analysis

Electric and Electrochemical Microfluidic Devices for Cell Analysis

Electrochemical Imaging of Cell Activity in Hydrogels Embedded in Grid-shaped Polycaprolactone Scaffolds Using a Large-scale Integration-based Amperometric Device

Electrochemical Biosensing System for Single Cells, Cellular Aggregates and Microenvironments

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