No abstract

Background:The updated guidelines by CDC (center for disease control) narrated the importance of using the dataprovided by hospital antibiogram for initial prescription of managing the infections. Therefore the current study had been planned to formulate the antibiogram in a tertiary care hospital of Islamabad. Objectives: To identify the local antibiotic susceptibility pattern against various isolates of urine pathogens.

To identify the susceptibility pattern of Fosfomycin in various clinical isolates by estimating the frequency in terms of percentages. A convenient sampling technique was adopted for study proceedings. Total 748(n) specimens for culture and sensitivity were received in the microbiology section of pathology department. Out of these 748(n) specimens, positive cultures were seen in 144(n). For culture and sensitivity proceedings, the recommended CLSI -2014 (clinical and laboratory standard institute) guidelines were followed. The bacterial isolation was done by biochemical tests. The zone diameter of >16 mm for 50µgm fosfomycin disc was considered as sensitive zone. While <15-12mm was considered as intermittent one and <12mm was the resistant zone. Data was recorded and analyzed by using SPSS version 20 for statistical inference. For numerical variables frequencies were calculated in terms of percentages. Seventy six 76.06% (n=143) gram negative and 68.42 % (n=13) gram positive organisms were sensitive to Fosfomycin. The efficacy of fosfomycin is more for gram negative (76%) as compared to gram positive organisms (68%).

This is an Open Access article distributed under the terms of Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any area, original work is properly cited.

The literature review is suggestive of good efficacy of Linezolid (Oxazolinedione derivative) against gram positive bacteria only. However it is deficient regarding its susceptibility in various clinical isolates. Therefore, the current study was planned to identify the susceptibility pattern of Linezolid in various isolates. Total 748(n) specimens were included in this study. Out of which 144(n) yielded positive growth. For microbiological culture proceedings all recommended CLSI – 2014 (Clinical and laboratory standard institute) guidelines were followed. The linezolid having 30µgm disc potency was used to assess its susceptibility. The clearing zone diameter of >21mm was considered sensitive. Data was recorded and analyzed by using SPSS version 20 for statistical inference. For numerical variables, frequencies were calculated in terms of percentages. The results of current study showed that 84.2% gram-positive and 45.6% gram-negative organisms were sensitive to linezolid. The efficacy of linezolid is more for gram-positive i.e 84.2% as compared to gram-negative 45.6%.