Background: Breast cancer is one of the common malignancies among women worldwide. Human papillomavirus (HPV) infections have been linked to many human cancers in addition to cervical cancer and one of them is breast cancer. Objective: To investigate the presence of human papilloma virus type 6 and type 11in breast cancer tissue specimens by in situ hybridization technique. Patients and Methods: Thirty four formalin-fixed, paraffin embedded tissue blocks from breast cancer patients were obtained from the archives of the pathology laboratory of Al-Yarmouk Teaching Hospital from January 2011 to July 2012. In addition formalin-fixed, paraffin embedded blocks tissue for twenty fibroadenoma of breast were collected and used as control group. In situ hybridization technique was used for the molecular detection of HPV type 6 and HPV type 11. Results: There were 82.4 % of breast cancer tissues positive for HPV type 6 and 88.2 % were positive for HPV type 11. Conclusion: The presence of human papillomavirus DNA in most breast carcinoma samples merits further investigation and confirmation by second assay in order to establish the exact role of this virus in the pathogenesis of breast cancer.
Cytogenetic analysis using G- banding technique was performed on bone marrow cells from a 22-year-old female who was diagnosed with Acute Lymphoblastic Leukemia . The chromosomal analysis showed a complex karyotype which included the Isochromosome 6(p) in 16 metaphases (16%) of 97 total metaphases studied . This finding suggests a strong selective pressure for loss of heterozygosity of genes located on 6q , and is in keeping with the hypothesis that one or more tumor suppressor genes might be located on the long arm of chromosome 6 . Moreover, trisomy (or tebasomy) for the short arm of chromosome 6, due to the isochromosome formation , indicating also a possible role for amplification of genes located on 6p in tumor progression in this case of acute lymphoblastic leukemia
Formalin-fixed , paraffin embedded blocks tissue for thirty one breast cancer patients were obtained from the archive of the Pathology laboratory of Al-Yarmouk Teaching Hospital from January 2011 to July 2012 . In addition Formalin-fixed , paraffin embedded blocks tissue for ten fibroadenoma of breast were collected and used as control group . These blocks were subjected to cut as serial thin sections of (4μm) thickness and were sticked on positive charge slides to be used for in situ hybridization for the detection of matrix metalloproteinase -2 in breast cancer (MMP-2) . Overexpression of MMP-2 was detected in 90.3% (28 out of 31) of breast cancer samples (> 50% of the cells appearing as positive) . The remaining 3 samples (9.7%) showed a weak- moderately expression (< 50% of the cells appearing as positive) . The statistical analysis demonstrated a highly significant differences in MMP-2 expression among patients with breast cancer when compared with fibroadenoma of breast patients (control group) . In conclusion MMP-2 plays an important role in pathpgensis of breast cancer and supports the evidence of its role in evolution , progression and cell survival of breast cancer .
Background: Breast cancer is one of the common malignancies among women worldwide. Human papillomavirus (HPV) infections have been linked to many human cancers in addition to cervical cancer and one of them is breast cancer.Objective: To investigate the presence of human papilloma virus type 6 and type 11in breast cancer tissue specimens by in situ hybridization technique.Patients and Methods: Thirty four formalin-fixed, paraffin embedded tissue blocks from breast cancer patients were obtained from the archives of the pathology laboratory of Al-Yarmouk Teaching Hospital from January 2011 to July 2012. In addition formalin-fixed, paraffin embedded blocks tissue for twenty fibroadenoma of breast were collected and used as control group. In situ hybridization technique was used for the molecular detection of HPV type 6 and HPV type 11.Results: There were 82.4 % of breast cancer tissues positive for HPV type 6 and 88.2 % were positive for HPV type 11.Conclusion: The presence of human papillomavirus DNA in most breast carcinoma samples merits further investigation and confirmation by second assay in order to establish the exact role of this virus in the pathogenesis of breast cancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.