Scientists are attempting to find novel methods to overcome cancers. Nanoemulsion systems as the novel drug delivery tools have been widely used in cancer therapy. In this study, the Carum Carvi oil nanoemulsions (CCONE) were prepared and its cytotoxic activity was studied on human colon cancer HT‐29 cells using MTT assay. Flow cytometry and Real‐time qPCR were triggered to evaluate the nanoemulsions' apoptotic properties. The results showed a significant negative association between the HT‐29 cancer cell viability and CCONE doses of treatments compared with Huvec normal cells (p value < 0.001). The IC50 values were estimated 12.5 µg/ml and 50 µg/ml for HT‐29 and Huvec, respectively. Moreover, we observed that increasing concentrations of nanoemulsions significantly upregulate Caspase‐3 gene expression. The results showed the CCONE is an efficient novel apoptosis inducer for human colon cancer cells without any undesirable side effects. However, further in vitro and in vivo researches are required.
Practical applications
Cancer is a complex and usually untreatable disorder. Several types of cancer therapy strategies have been applied widely to overcome cancers. Chemotherapy has been used in various types of cancers. In most cases, not only it had not been effective on cancer cells but also been distractive within normal tissues. According to results, Carum Carvi essential oil nanoemulsions have apoptotic and cytotoxic effects on colon cancer cells (HT‐29). When it comes to cancer of any kind, it's important to realize that no dietary supplement can fully treat, cure, or prevent cancer. However, there are some supplements that can potentially decrease the risk of cancer. Nanoemulsions present several advantages including the ability to incorporate hydrophilic, amphiphilic, and lipophilic excipient ingredients, high physical stability, and rapid gastrointestinal digestibility. The Carum Carvi essential oil nanoemulsion can also be applied as an effective food supplement due to its potent apoptotic activity.
Myricetin is a flavonoid with anticancer properties. This study aimed to formulate myricetin in the form of solid lipid nanoparticles (SLN), decorated with chitosan (CS) and active-targeted with folic acid (FA). After characterization, the in vitro release, cytotoxicity, antioxidant, and ability of the formulation to induce apoptosis using flow cytometry, fluorescent microscopy, and real-time qPCR were examined. Then in vivo anti-angiogenesis on chick chorioallantoic membrane (CAM) and antitumor activities on mice bearing tumor models were investigated. The present study showed that the size of 310 nm and zeta potential of + 30 mV were acceptable for oral administration. The Michaelis–Menten model fitted the drug release pattern with lag during 144 h of the study. The cytotoxicity assay showed that myricetin-SLN-CS-FA significantly killed cancer cells at the concentrations of 6.25, 12.5, 25, 50 and 100 µg/mL (*p < 0.05, **p < 0.01, and ***p < 0.001). The highest level of apoptosis was shown at the concentration of 45 µg/ml in flow cytometry, and fluorescent studies. These results showed the anticancer properties of myricetin-SLN-CS-FA in a dose-dependent manner. The real-time results also indicated that the formulation exerted its cytotoxic effect by activating apoptosis genes. The DPPH, ABTS, and FRAP studies also demonstrated the significant antioxidant properties of the myricetin-SLN-CS-FA (*p < 0.05, **p < 0.01, and ***p < 0.001). The anti-angiogenic activities of the formulations depicted in the CAM assay significantly decrease the number and length of the vessels (*p < 0.05, **p < 0.01, and ***p < 0.001), and also affect VEGF and VEGFR, genes involved in angiogenesis (**p < 0.01, and ***p < 0.001). The antitumor studies indicated the statistically significant effects of myricetin-SLN-CS-FA on reducing tumor volume (*p < 0.05 and ***p < 0.001). The H&E staining of the liver and monitoring of the animal weights also indicated the safety of the formulation. The analysis of mRNA expression in liver and tumor demonstrated that myricetin-SLN-CS-FA exerts its antitumor activities by modulating the inflammatory and oxidative responses in the tissues.
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